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- Suppose you inoculated two 0.4% semi-solid agar deeps: one deep was inoculated with a motile organism and the other deep with a non-motile organism. You (in a stroke of genius) found a way to quantitatively analyze the deeps using a spectrophotometer. Which deep would have the highest absorbance and why?You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell density of this culture?POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What is your observation from potato A to D? 2. What can you conclude by comparing potato A and potato B? 3. What can you conclude by comparing potato A and potato C?
- POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What can you conclude by comparing potato A and potato D? 2. The liquid in the cavity of some potatoes came from where? 3. What is your conclusion about the experiment?microbiology: Aseptic Culture Techniques Lab Explain why a loop or needle is flamed before it is dipped into a tube containing a pure culture. Also, explain why it is flamed after an inoculation is completedEXPERIMENT : REMOVAL OF WATER FROM MICROBIAL CELLS USING FREEZE DRYING METHOD Objective To learn on the technique of freeze drying for microbial sample preparation and preservation. Result Weight of test tube (g) -Wi 15.34 Weight of test tube with cells (g) - Ww 34.65 Amount of water removal = weight of test tube with cells – weight of test tube = 34.65 g – 15.34 g = 21.16 g QUESTION: Discuss the result and explain the freeze drying method that used in this experiment.
- You perform a set of serial dilutions using 99-mL water blanks (successive 1-mL dilutions into blanks A, B, and C, respectively). Following spread plating and incubation of a 0.1 mL subsample from Blank C, you count 66 colonies on the plate. How many colony-forming units are in the original broth culture?EXPERIMENT : REMOVAL OF WATER FROM MICROBIAL CELLS USING FREEZE DRYING METHOD Objective To learn on the technique of freeze drying for microbial sample preparation and preservation. Result Weight of test tube (g) -Wi 15.34 Weight of test tube with cells (g) - Ww 34.65 Amount of water removal = weight of test tube with cells – weight of test tube = 34.65 g – 15.34 g = 21.16 g QUESTION: Discuss the result and explain the freeze drying method that used in this experiment how the freeze drying methodDesign a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solution
- Which of the following sample(s) is NOT expected to cause a SUBSTANTIAL decrease in absorbance over time when performing light scattering experiments with bacteria? [Choose the best possible answer] LOAD and Bacteria alone Lysozyme standards HEW and CARB1 Bacteria aloneYou have spread 0.1ml of a 1x10-8 diluted bacterial culture sample on a Petridish and counted35 colonies. What was the cell density of your original culture (in cells/ml)? How many cellsdid you have in 100ml of that culture? DON’T FORGET TO ROUND!4. You used 1mL of a river water sample at a 10-3 dilution and added this to an agar plate. After incubation, you count 60 colonies. How many colony forming units (CFUs) are present? Show your work.