Using the Beer's Law plot constructed for TZ at 430 nm, please report the effective molar absorptivity (ε*, L/μmol), which is the slope of the linear trendline through the data. €*, L/μmol Unrounded 3 Rounded

Using the Beer's Law plot constructed for TZ at 430 nm, please report the effective molar absorptivity (ε, L/μmol), which is the slope of the linear trendline through the data. €, L/μmol Unrounded 3 Rounded

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter93: Refractometry

Section: Chapter Questions

Problem 1P

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Question

heres the data

TZ #	Concentration	Absorbance at 430 nmnm
1	33.6480	0.931
2	25.2360	0.757
3	16.8420	0.210
4	8.41200	0.137
5	4.20600	0.122

Using the Beer's Law plot constructed for TZ at 430 nm, please report the effective molar absorptivity (ε*, L/μmol), which is the slope
of the linear trendline through the data.
ε*, L/μmol
Unrounded
3
Rounded
3

Absorbance
0.8
0.6
0.4
0.2
5
Beer's Law Plot of TZ at 430 nm
10
15
430 nm: y = 0.0259825 x
20
Concentration (μM)
25
30
35

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Step 1: Introduce to Beer's law

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Step 2: Introduce to the concept of calibration curve

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Step 3: Graphical plot of concentration vs Absorbance

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Step 4: Finding effective molar absorptivity

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