Various chemical methods can be used to permeabilize or lyse cells including the use of enzymes. List three important factors to consider when developing a lysis method using enzymes.
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Various chemical methods can be used to permeabilize or lyse cells including the use of enzymes. List three important factors to consider when developing a lysis method using enzymes.
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Estimate the osmotic pressure drop across the membrane of an animal cell in a 0.10 M NaCl solution, assuming that the internal total solute concentration is 0.36 osM and temperature is 30.0°C. Do you think that this pressure drop would lyse cells? Why or why not?
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- The primary function of the kidney is to exchange molecules across a membrane between the blood and the urine. One type of kidney cell has a basic rectangular shape, except for a single surface, which is lined with tiny, finger-like projections that extend into the surrounding extracellular space. Which of the following best explains the advantage these projections provide the cell? The projections increase the selectivity of the membrane because the small size of the projections limits the number of transport proteins that can be embedded in the membrane. The projections increase the volume of the cell without affecting the surface area, which increases the metabolic needs of the cell. The projections increase the surface area–to-volume ratio of the cell, which allows for more efficient nutrient exchange with the environment. The projections increase the speed at which an individual molecule can move,…The solutions in the two arms of the U-tube are separated by a membrane that is permeable to water and glucose but not to sucrose. Side A is filled with a solution of 2.0 M sucrose and 1.0 M glucose. Side B is filled with 1.0 M sucrose and 2.0 M glucose. Initially, the solution in side A, with respect to that in side B, is what? After the system reaches equilibrium, what changes are observed?In this part of the assignment, assume you have carried out the microscopic examination of the effects of osmosis in plant cells experiment The image in Figure 4 is of Elodea canadensis leaf cells in isotonic solution (normal saline). The image in Figure 5 is of Elodea canadensis leaf cells in 10% sucrose solution. The image in Figure 6 is of Elodea canadensis leaf cells in distilled water. a. Determine cell size and vacuole size. b. Calculate the percentage change in vacuole size for each treatment compared to the control. Use the formula: Experimental Size - Control Size/Control Size c. Based on these results, explain the tonicity of each treatment using experimental results to support your answer as would appear in the discussion section of a formal report (maximum four (4) lines). d. Was the percentage change in vacuole size for the experimental conditions relative to the control of similar magnitude? If the change was not of the same magnitude, explain the difference in effect…
- Two to three drops of mouse blood samples were placed in three different vials containing 0.07 M NaCl,0.15 M NaCl, and 0.30 M NaCl. A drop from each of the three vials were obtained and put on a slide forobservation under HPO. The effects of the different osmotic concentrations on the cells are shown in figures in your worksheets. Label the cell membrane for each figure. Give a short description (size and cell shape) for each of the RBC samples on the space provided in your worksheet. Compare their appearances with RBCs in the blood smear. Use the following guide questions in providing descriptions for each item. Which preparation has cells that look similar as those in the blood smear? What does this indicate about the movement of water in the cells? In which solution do the cells appear differently from the normal RBCs? What part of the cell could have possibly controlled such movement of water? What is its property that allowed this movement?Here is a chloride cell in the gill epithelium of a fish. For reference, NKA = Na+/K+ ATPaseNKCC = Na+/K+/Cl- cotransporter. CFTR allows for movement of Cl- from inside the cell out into the water down a concentration gradient. Which of the following would be true of CFTR? A. It is a primary active transporter. B. It is a symporter. C. It is a secondary active transporter. D. It is a channel that allows for facilitated diffusion.A cell with a K+1 concentration of 0.3 eq/L, and a Na+1 concentration of 0.1 mol/L is placed into a solution with a K+1 concentration of 0.2 eq/L, and a Na+1 concentration of 0.2 mol/L. It is assumed that the K+1 and Na+1 can both pass through the cell membrane (the K+1 and Na+1 transport proteins are open). Answer the following true or false questions. 1. Diffusion of a solute through the membrane is called dialysis 2. K+1 will dialyze (diffuse) out of the cell 3. Na+1 will dialyze (diffuse) into the cell 4. Like the diffusion of gases, diffusion of particles in a liquid is independent of the other solutes
- If a percent difference is a negative number, what does this tell us about the change in weight of the artificial cell? If the percent difference is a negative number, what does this tell us about the movement of water across the membrane?If you made a hematocrit in two tubes. Tube A is made of 0.60M NaCl, and Tube B is made of 1.00M of NaCl, describe how the cell would respond in these solutions? Which would have a higher rate of osmosis? If the cell is permeable to NaCl, which would have a higher rate of diffusion?Explain how salt concentrations contribute to the cell membrane intergrity in cells? What are the three different scenarios that could occur and why?
- how can osmosis be applied to the physiological principle of a controlled exchange of materials occurs between compartments and across cellular membranes?uring dialysis, a semi-permeable membrane with a molecular weight cutoff of 10,000 is used. Select the true statements from the list below (more than one answer could apply) Group of answer choices A monoclonal antibody with a 150 kDa MW is retained and does not diffuse across the membrane A small enzyme with 20 kDa MW diffuses readily across the membrane Buffer salts with molecular weights ranging between 100 and 500 Da diffuse across the membrane until equilibrium (equal concentration) is reached on both sides of the membrane None of the above statements are correct Please answer asapList and explain the four functions of the basic components in a flow cytometer? How is a FACS machine different? Please give an example of how a flow cytometer or FACS can be used to identify two different cell populations.