n this part of the assignment, assume you have carried out the microscopic examination of the effects of osmosis in plant cells experiment The image in Figure 4 is of Elodea canadensis leaf cells in isotonic solution (normal saline). The image in Figure 5 is of Elodea canadensis leaf cells in 10% sucrose solution. The image in Figure 6 is of Elodea canadensis leaf cells in distilled water. a. Determine cell size and vacuole size. b. Calculate the percentage change in vacuole size for each treatment compared to the control. Use the formula: Experimental Size - Control Size/Control Size c. Based on these results, explain the tonicity of each treatment using experimental results to support your answer as would appear in the discussion section of a formal report (maxi
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
In this part of the assignment, assume you have carried out the microscopic examination of the effects of osmosis in plant cells experiment
The image in Figure 4 is of Elodea canadensis leaf cells in isotonic solution (normal saline). The image in Figure 5 is of Elodea canadensis leaf cells in 10% sucrose solution. The image in Figure 6 is of Elodea canadensis leaf cells in distilled water.
a. Determine cell size and vacuole size.
b. Calculate the percentage change in vacuole size for each treatment compared to the control. Use the formula:
Experimental Size - Control Size/Control Size
c. Based on these results, explain the tonicity of each treatment using experimental results to support your answer as would appear in the discussion section of a formal report (maximum four (4) lines).
d. Was the percentage change in vacuole size for the experimental conditions relative to the control of similar magnitude? If the change was not of the same magnitude, explain the difference in effect between the two treatments.
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