Restriction mapping of the delta chromosome I need help with question two please H.A E SAEAS23,130 BP9,416 BP6,557 BP4,361 BP2,322 BP2,027 BP564 BPIdeal Gel2. Linear DNA fragments migrate at rates inversely proportional to thelog,, of their molecular weight. For simplicity's sake, base-pair lengthis substituted here for molecular weight. Use the photograph of yourstained gel and a ruler to construct a graph that relates the base-pairsize of each restriction fragment to the distance migrated through thegel. The matrix on the next page gives the size in base pairs (BP) of ADNA fragments generated by the HindIII digest:11I the A ChromosomeHindlllApalBPEcoo1091DDSnaBIApal/EcoO1091Apal/SnaBIBP23,130BPDBPBPВРD]9,4166,5574,3612,3222,027564125*.usually runs off the end of the mini-gel during electrophoresisa. Orient your gel photo with the wells at the top, and locate one ofthe two H lanes. Working from top to bottom, match the base-pair sizes of the HindIII fragments with the bands that appear inthe H lane. Carefully measure the distance (in mm) that eachHindIII fragment migrated from the sample well. Measure fromthe front edge of the well to the leading edge of each band. Enterthe distance migrated by each fragment into the D column.b. Set up semilog graph paper using the distance migrated as the x(arithmetic) axis and the base-pair length as the y (logarithmic)axis. (The first cycle of the y axis should begin with 1,000 bp andend with 10,000 bp–counting in 1,000-bp increments. The sec-ond cycle begins with 10,000 bp and ends with 100,000 bp–counting in 10,000 bp increments.) Then, plot the distancemigrated versus base-pair length for each HindIII fragment.Finally, connect the data points.c. Measure the distance migrated by the smallest Apal restrictionfragment, and locate this distance on the x axis of your graph.Then, use a ruler to draw a vertical line from this point to its inter-section with the HindIII data line. Now, extend a horizontal linefrom this point to the y axis. This gives the bp size of the smallestApal fragment. Enter the result into the matrix above. Do not cal-culate the length of the largest fragment at this time.d. Repeat step c to calculate the bp sizes of the smallest restrictionfragments in the EcoO1091 and SnaBI single digests and the twosmallest fragments in the double digests. Enter the data into thematrix above.e. The gel does not provide an accurate determination of the sizes ofthe largest fragments in each of the digests, because they are out-side the linear resolving range of the gel. Therefore, determine thelength of the largest fragment in each digest by subtracting thelength(s) of the smaller fragment(s) from the length of the Agenome (48,502 base pairs). Enter the data into the matrix above.3. Construct a restriction map of the cutting sites of 2 DNA, showingthe cutting sites for Apal, EcoO1091, and SnaBI.a. The data from the single digests indicates how far each enzymecuts from the end of the 2 chromosome.

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the mean size is close to 4000 bp. DNA fragments of this length are useful in the lab, since…