What does autoradiography reveal about cells actively expressing genes and what does this indicate?

Q: What is ex vivo gene therapy?

A: Gene therapy is the bioengineering technique in which any genetic defect is corrected by altering…

Q: What is ORF scanning?

A: The open reading frame(ORF) in molecular genetics is a continuous stretch of codons that begins with…

Q: How can we isolate stem cells and how can we use these cells in the clinic?

A: In this question, we have to answer how to isolate stem cells and how we can use these cells in the…

Q: What activities does the Genetic Information Nondiscrimination Act prohibit?

A: The Genetic information non-discrimination act (2008) is a law that defend the individuals from the…

Q: Does use of nucleic acid probes depend on gene expression?

A: Introduction: Nucleic acid hybridization or nucleic acid probe are used to determine specific gene…

Q: What is the difference between gene therapy and cell therapy?

A: A therapy, also known as a medical treatment, is an attempt to address a medical problem after a…

Q: What are advantages of genetic test?

A: Genetic Testing: The changes in chromosomes, genes or proteins are being identified via methodology…

Q: Explain gene-knockout technique.

A: According to the question, we have to provide an explanation of the gene knockout technique.  So,…

Q: What are the application areas of molecular markers?

A: Molecular marker is also called as genetic markers which are DNA fragments present at specific…

Q: What is the advantage of using stem cells for genetherapy or gene editing?

A: Gene therapy is the process of replacing the abnormal gene with a normal copy of a gene for the…

Q: What is molecular DNA markers?

A: DNA is a polymer of deoxyribonucleoside monophosphates (dNMP) covalentlylinked by…

Q: Explain the process of “blue-white” screening ?

A: Blue-white screening is a technique which allows proper detection of recombinant bacteria for…

Q: explain why gene mapping is important and how this technique can be used in disease investigation?

A: The Human Genome Project aimed at developing tools to identify new genes and their function. Genetic…

Q: What are Gene-knockout experiments?

A: Gene-knockout:              A genetic technique in which one of an organism's genes is made…

Q: What is double-knockout animals (DKOs) ?

A: Gene is the basic unit of heredity of an organism. In most animals, Deoxyribonucleic acid (DNA) is…

Q: How can AI and ML be used to identify biomarkers?

A: AI stands for Artificial Intelligence while ML stands for Machine Learning. Machine learning is a…

Q: How to identify all the somatic mutationswithin tumors ?

A: A somatic mutation is a modification within the desoxyribonucleic acid sequence of a somatic cell of…

Q: How to use a microarray for transcriptome analysis ?

A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…

Q: How is autoradiography used to detect a mutated gene?

A: Autoradiography is a technique of imaging that uses radioactive substances contained in the exposed…

Q: How does Pol II recognize the correct DNA to transcribe?

A: RNA polymerase II is a multiprotein complex that transcribes DNA into precursors of messenger RNA…

Q: What is the difference between somatic gene therapy and germ-line gene therapy?

A: Gene is a functional unit of heredity. A gene is a sequence of nucleotides in genome that codes for…

Q: What are knockout mice, how are they produced, and for what are they used?

A: The gene knockout is a mechanism in which one of the genes of an organism is made inoperative. The…

Q: How is DNA microarray technology used to determine the differences in gene expression?

A: A Deoxyribonucleic acid (DNA) microarray is a collection of microscopic DNA spots attached to a…

Q: What is the role of a vector in gene therapy?

A: Gene therapy is based on the premise that replacement of a disease-causing gene with the healthy…

Q: What is preimplantation genetic diagnosis?

A: Prenatal diagnosis is performed for the identification of genetic diseases and their predisposition…

Q: What are the challenges faced while identification of novel biomarkers?

A: Biomarkers are the measurable indicator for severity or pressure of some stages of diseases.…

Q: How can genetic testing help in cancer diagnosis? Briefly explain

A: Cancer is a group of disease which involves abnormal growth of cell with the ability to spread to…

Q: What class of genes is used in MLST analyses?

A: MLST refers to multilocus sequence typing. MLST involves higher frequency of recombination in…

Q: What are the Methods of therapeutic gene delivery?

A: Gene therapy is a promising method for correcting host pathology by the manipulation of DNA…

Q: What is gene sequencing used for?

A: DNA sequencing is the process of determining the sequence of nucleotide bases that make up a DNA.…

Q: Why is it necessary to examine gene-expression profiles, in additionto genome sequences, for…

A: Gene expression profiling is the analysis of the behaviour of thousands of genes at once to…

Q: What is clinical exome test?

A: The exome is the part of the genome which contain all the protein coding sequences.

Q: What is a notch 3 gene mutation test?

A: Notch is a cell surface receptor that receives and transmits signals in notch signalling pathway. It…

Q: What are stem cells, and what safety mechanism protects them from mutation?

A: Stem cell  Stem cells are pluripotent cell that is produced by bone marrow cells.

Q: Was Biological condition is needed for in-vitro environment?

A: In-vitro:- it means outside the living organisms. For example any biological experiment or reactions…

Q: What are imaging biomarkers?

A: A biomarker is a biological characteristic that is measured and evaluated as an indicator of a…

Q: What is Northern blot analysis ?

A: The nucleic acids transfer on a solid phase membrane for identification is called the blotting…

Q: How is DNA microarray used in detecting cancer?

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: What are the advantages of molecular diagnostics over conventional methods?

A: Disease: -any discomfort, disability, or impairment in the health caused due to functional or…

Q: For what the first clinical gene therapy was given?

A: Gene therapy is a medical technique in which genes are used for treating and preventing a disease.…

Q: What is gene targeting in embryonic stem cells?

A: Introduction Gene targeting in the embryonic stem cells through homologous recombination techniques…

Q: What is a gene gun used for?

A: Step 1 A gene gun or a biolistic particle delivery system is a device that can transform almost any…

Q: What is selectable marker gene?

A: The microbial culture technique is the method of multiplying the microbial organism under controlled…

Q: From microarray analysis how do we know what genes are being expressed in a specific tissue?

A: The laboratory tool that is used to detect the expression of many genes simultaneously. DNA…

Q: What is probe? Why it is used in the library screening ?

A: Biotechnology is the practice of using living organisms such as animals, plants, microbes, and…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.