Q: Why is plasmid useful for DNA transfer?
A: A plasmid is circular, extrachromosomal, small piece of DNA present in the cytoplasm that is…
Q: What functions must a plasmid encode to be self-transmissible?
A: A plasmid that codes for its own mating pair formation (MPF) genes is called self transmissible or…
Q: How is the copy number of plasmid vector and yield of the recombinant protein related to each other?
A: In biotechnology, plasmid refers to the double stranded, circular DNA of bacteria; when these…
Q: Why was it necessary to soak the gel in hydroxide before performing the blot?
A: Gel electrophoresis is a technique which is used to separate the molecules based on the size of the…
Q: What role does plasmids play in genetic engineering?
A: A plasmid is a small, circular, double-stranded extra chromosomal DNA molecule present within the…
Q: What is Ti plasmid ?
A: The biochemical molecule that is built up with “two polynucleotide chains” is called…
Q: What does the F plasmid code for?
A: The plasmid is defined as a small, circular, extra-chromosomal, double-stranded DNA molecule present…
Q: The F plasmid encodes which two functions essential for conjugation?
A: Plasmid encodes the Synthesis of F pilus and several other proteins required for Conjugation. Plasma…
Q: What are recombinant plasmids ?
A: Plasmids are circular, double-stranded containing an extrachromosomal DNA molecule.
Q: What does the acronym CRISPR stand for?
A: CRISPR is a technique used in editing of base pair of genes. It is important tool in genetic…
Q: What would happen if a plasmid without a selectable marker was chosen as a cloning vector?
A: DNA cloning is a type of cloning method in which a large number of DNA molecules from a single…
Q: What different methods were used to help break the genetic code? What did each method reveal and…
A: The genetic code is a triplet code, in which three nucleotides encode each amino acid in a protein.…
Q: Why might it be advantageous for a bacterial cell to receive the F plasmid from another cell?
A: The F plasmid, the fertility factor is a large plasmid that consists of genes that allow the…
Q: What is the purpose of arabinose in the pGLO lab, where is arabinose located, how can it be…
A: Arabinose acts as an allosteric regulator of AraC changing and tells which DNA sites it binds to and…
Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…
A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…
Q: What is a restriction enzyme? How can restriction enzymes be used to splice a piece of human DNA…
A: BASIC INFORMATION ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…
Q: Why do bacteria make restriction endonucleases?
A: Deoxyribonucleic acid (DNA) is a double-stranded molecule, which consists of two strands of…
Q: Why do eukaryotic cells do not contain restriction enzymes?
A: Restriction enzymes are used extensively in molecular biology to cut DNA. One of the unique features…
Q: What normal role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from…
A: A restriction enzyme is a protein that recognizes a particular DNA sequence and cuts the DNA at a…
Q: Can you separate a plasmid from a non plasmid nucleic acid? How?
A: Plasmids are usually the circular, extrachromosomal DNA that has the ability of self replicating…
Q: Why are plasmids used as vector for DNA Recombination? What other vectors can be used?
A: Plasmids are the extrachromosomal DNA that can replicate independently. They contain an origin of…
Q: Where is the F plasmid located in an Hfr cell? Where is the F plasmid located in an F+ cell?
A: Prokaryotic cells are unicellular organisms that differ from multicellular organisms in composition…
Q: which parts would a plasmid vector with 2500 bp have in E. coli
A: A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's…
Q: What is a restriction fragment?
A: A restriction fragment can be defined as the amount of DNA fragment which occurs due to the cutting…
Q: What are the essential elements of a plasmid cloning vector? Discuss the importance of each element…
A: Essential elements of plasmid cloning vector Presence of Origin of replication for autonomous…
Q: In producing genetically engineered human insulin in bacteria, why is it important to use the same…
A: In the production of human insulin by bacterium, E.coli, the human insulin gene is introduced into…
Q: What is the importance of the antibiotic resistance marker gene in the plasmid.
A: ANSWER;- - Adding an anti-microbial obstruction gene to the plasmid tackles the two issues on the…
Q: How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?
A: Alkaline lysis is the most common method used for separating plasmid DNA from chromosomal DNA.
Q: If a F plasmid does not have the gene encoding TraJ, this F plasmid would
A: Conjugation is a major mechanism for dissemination of antibiotic resistance and virulence among…
Q: What happens to YAC • Plasmids with origins of replication and a centromere but no telomeres?
A: Answer: Introduction: Yeast artificial chromosomes (YACs) are chromosomes produced by genetic…
Q: What is a restriction digest? What does it mean if you were given a precut DNA?
A: Restriction enzyme digestion takes advantage of naturally occurring enzymes that cleave DNA at…
Q: Why are plasmids dangerous?
A: It is a small, circular, double-stranded DNA molecule which naturally exist in bacterial cells, and…
Q: What is CRISPR?
A: Introduction Bacteria and viruses are tough competitor to each other, in order to overcome each…
Q: What is a plasmid and what is its function?
A: Prokaryotes are the single celled organisms (unicellular) and are the simplest form, which do not…
Q: What is the Ti plasmid and how is it modified for the genetic modification of plants?
A: Plasmids are circular and extrachromosomal DNA (sometimes RNA) present in prokaryotic as well as…
Q: Besides plasmid, what other vectors could you use to amplify your gene of interest? Name THREE (3)…
A: A vector is any vehicle, often a virus or a plasmid that is used to ferry a desired DNA sequence…
Q: What is the role of ‘Ori’ in any plasmid?
A: The ori is where DNA replication starts, enabling a plasmid to repeat itself as it must to get by…
Q: Why is the DNA stored cold with the InstaGene matrix after boiling the samples?
A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…
Q: What is RNA primer?
A: The nucleic acid is a nucleotides chain that stores genetic information. It forms RNA and DNA that…
Q: the role of X-gal? What color will cells with the plasmid be in the absence of X-gal? Why?
A: X- gal refers to the organic compound which comprises a galactose associated with an indole group.…
Q: Why are plasmids used for transformation?
A: Transformation is the genetic alteration in a cell by direct uptake and incorporation of exogenous…
Q: What do plasmids contain?
A: Plasmids are extra-chromosomal DNA that provides an added advantage to the bacteria. Plasmids are of…
Q: What are palindromes, and how are they related to restriction endonucleases? Why are they useful for…
A: The two strands of polynucleotides that make up the DNA molecule are intertwined in a helical…
Q: What is the purpose of ampicillin in the pGLO lab, where is the ampicillin locates, how it can be…
A:
Q: What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic…
A: Biotechnology is innovation that uses natural frameworks, living beings or parts of this to create…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
