What factors determine the relative intensity of transitions in a pure rotational absorption spectrum? When will a maximum intensity be observed?
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- 1 . What is the absorption peak(s) of bromocresol green? Make sure to write down the wavelength at which the absorption peak(s) occur. Include units, please 2.Are the absorption spectrums and peak(s) for methyl orange and bromocresol green the same? Why or why not?What is the absorption peak(s) of methyl orange? Make sure to write down the wavelength at which the absorption peak(s) occur. Include units, please What color(s) does methyl orange absorb the best?What is the absorption peak(s) of methyl orange? Make sure to write down the wavelength at which the absorption peak(s) occur and include units What color(s) does methyl orange absorb the best?
- The magnesium electron spectrum has a line at 266.8 nm. Does it have a greater speed in a vacuum (an area without an atom or particle) than does red light with a wavelength of 652 nm? Why or why not?What functional unit vibration would you expect to see on an IR spectrum and about where would it absorb (1/cm) if there were a significant concentration of the isopentyl alcohol reactant in an impure isopentyl acetate sample made by our procedure that would distinguish it from isopentyl acetate?1. What is a “dNTP” and why are they included in the reaction mixture? 2. What will be the final concentration of 25 mM MgCl2, 5 mM dNTPs, and each 25 µM primer in one of the reaction tubes with the final volume of 50 microliters? Hint: use the information in the left column of Table 1 to help you with these calculations. (For one reaction) item 5.25x master mix – add things in order 32.7 µl ddH2O 171.7 µl 10 µl 5x reaction buffer 52.5 µl 3 µl 25 mM MgCl2 15.75 µl 0.5 µl 5 mM dNTPs 2.63 µl 1 µl 25 µM “forward” primer (#3012) 5.25 µl 1 µl 25 µM “reverse” primer (#3013) 5.25 µl 0.8 µl thermostable polymerase 4.2 µl (do not add until needed) Total: 49 µl Total: 257.3 µl
- The wavelength for cobalt (II) chloride is set to 549 nm. Why is this wavelength being used?Enter the correct color for the visible light for each wavelength. 380-450 450-495 495-570 570-590 590-620 620-750 From the above wavelengths, which has the highest amount of energy?What might happen if you omit potassium tellurite in using GCM?