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What is it called when you stab the nutrient agar during the inoculation step of isolating a microorganism?
Step by step
Solved in 2 steps
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
- If you suspect that your microbial culture growing in nutrient broth is contaminated, what can you do to confirm this?Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?What is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?
- Why is dilution a necessary part of pure culture preparation?How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long should you wait for bacterial colonies to grow on agar plates? What is the appropriate temperature?.What is the most difficult type of microbial cell or structure to kill? What methods can be used to sterilize a sample contaminated with these?