Q: What is the difference between a mixed culture and a contaminated culture if they both contain more…
A: In microbiology , a bacterial culture is a growth of microorganisms which have been grown in a…
Q: What types of samples can be measured with Circular Dichroism? Can information be extracted?
A: Circulate dichroism is the difference in the absorption of left handed circularly polarised…
Q: Illustrate the step by step method of preparing culture media.
A: Introduction Culture medium:- It is a liquid or gel designed for specific mixtures of nutrients and…
Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…
A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: Why does motility media contain a lower amount of agar than ordinary agar?
A: Agar is a phyco-colloid polymer of galactose monomers obtained from red algae (Gelidium and…
Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…
A: Generation time is the time which is taken by the bacteria to double in number during a specified…
Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…
A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…
Q: What is agar and why is it necessary for growing bacteria on plates?
A: Agar is a mixture of two polysaccharides- agarose and agaropectin. It is derived from red-purple…
Q: Why is the culture medium referred to as a highly enriched laboratory soil?
A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…
Q: aseptic technique and pure culture technique
A: ASEPTIC TECHNIQUE_ *Inserting PICC lines. *Performing dialysis. *Inserting catheters. *Running…
Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…
A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…
Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: What is the difference between the pour plate method and the spread plate method? Why might you…
A: Standard plate count method is a growth based approach that counts the number of living…
Q: What is a pure culture?
A: Introduction Culture is referred to th process of growing the cell outside the animal or plant body…
Q: Microbiology
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…
A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: What concentration of agar is used in a semisolid medium for motility determination?
A: Motility is a feature characteristic of only some bacteria. This ability allows the bacteria to move…
Q: Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?
A: There are several parameters which need to be studied in microbiology about different…
Q: why it is useful to plot the microbial growth from a growing microbial culture?
A: The term microbial growth refers to the growth of a population of microbe, it is an increase in the…
Q: What concentration of agar is used in a semisolid medium for motility determination? How does that…
A: Agar is a polysaccharide that is obtained from marine seaweeds. Agar is mostly used in food and…
Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…
A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…
Q: Why is the time of exposure and temperature required for sterilization greater for dry heat than for…
A: Any technique that removes, destroys, or deactivates all forms of life and other biological agents…
Q: What is differential medium and differential media? Explain the difference ?
A: Difference between differential medumium - .- A differential medium support the expansion of any…
Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.
A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…
Q: Why is PBS buffer preferred when preparing a monolayer cell culture?
A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…
Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?
A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…
Q: What is a pure culture and how can one be obtained
A: Cells are the structural and functional units of life.
Q: Are TSA, MRS and MOX differential media? Why or why not?
A: In microbial biology, differential media refers to any growth media that comprises of certain…
Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…
A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…
Q: Can culture media be sterilized in the hot air oven? why or why not?
A: Sterilization is the process of deactivating, killing, or removing any kind of microorganism (or…
Q: What is the main difference between plates before and after centrifugation? What may be the purpose…
A: Answer: CENTRIFUGATION = It is the process involving mechanical force that is centrifugal force,…
Q: What is the nature of samples that should NOT be placed inside the drying-oven?
A: According to the question, we have to explain the nature of the samples that should not be placed…
Q: What are the culture media that can be used for cultivating non-specific and specific…
A: A microbiological culture medium is a substance that promotes the growth, support, and survival of…
Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…
A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…
Q: Why is egg enrichment not added to BPA prior to sterilization?
A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…
Q: What is diluent? Why is it useful for working with bacterial samples?
A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…
Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: What is the meaning of the word sterile? What would happen if culture media freshly prepared were…
A: Sterile It refers to the state of being free from any bacteria or any other living microorganisms.…
Q: What are the primary uses of the following preparations of culture media? Agar regular slant Agar…
A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…
Q: Why should agar media be completely dissolved before they are dispensed in tubes and plates? What…
A: Introduction A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: What is this media? What type of media is it? Which type of organism can grow on this plate?…
A: Media is... Agar This is the solids type of Media. Organism type... Streak plate technique is used…
Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…
A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml
Why is dilution a necessary part of pure culture preparation?
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- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?What is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?
- In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?
- Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?If cultures are suffering severely due to alkaloid production and accumulation in the medium then, how can i overcome this problem?What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?