What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can inoculation loops and needles be sterilized by autoclaving? Explain When is sterilization by filtration preferred over sterilization by heat? Why?
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- What is the hazard of the splattering tendency in flame sterilizing an inoculating loop?
- Can inoculation loops and needles be sterilized by autoclaving? Explain
- When is sterilization by filtration preferred over sterilization by heat? Why?
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- Compare and contrast antiseptic and disinfectant. What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can inoculation loops and needles be sterilized by autoclaving? Explain.When is sterilization by filtration preferred over sterilization by heat? Why? What makes heat an effective sterilizing agent? Why are the conditions required for sterilization greater for dry heat (160-180°C for 1-2hrs) than for steam (121°C for 15min)?Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that hinder effective sterilization through autoclaving? When can you say that the sterility of an instrument is compromise?
- What is the point of the pressure in the autoclave sterilization?Can culture media be sterilized in the hot air sterilizer? Why or why not?how do microorganisms such as bacteria eliminate harmful reactive oxygen species? Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.
- Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?In autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and pressure at 15 psi for 15 to 20 min? What will happen if this was done in only for 10 min? Can you autoclave lab materials and decontaminate cultures at the same time?
- Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when performing the oxidase test in microbiology lab?What is the effect of moist heat and dry heat? Define TDT and TDP. What is the minimum sterilization condition of autoclave? What is tyndallization, incineration, pasteurization.Why is chlorination, although beneficial in terms of bacterial pathogen control, of environmental concern?