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- A chemist wanted to test his hypothesis that the disulfide bridges that form in many proteins do so after the minimum energy conformation of the protein has been achieved. He treated a sample of an enzyme that contained four disulfide bridges with 2-mercaptoethanol and then added urea to denature the enzyme. He slowly removed these reagents so that the enzyme could re-fold and re-form the disulfide bridges. The enzyme he recovered had 80% of its original activity. What would be the percent activity in the recovered enzyme if disulfide bridge formation were entirely random rather than determined by the tertiary structure? Does this experiment support his hypothesis?Designate the chiral centers of the following molecules and show which one of them is achiral?Specify the configurations (R or S) at ALL chiral centers in the molecules below. Please note that some molecules below may have more than 1 chiral centers, and you are responsible to find all the chiral centers.
- What is the relationship between these molecules? Enantiomers, Diastereomers, IdenticasConsider the formation of a dimeric protein 2P ->P2 At 25.0 °C , the enthalpy of the reaction is 17 kj/mol and the entropy of the reaction is 65 j/kmol. Is the dimerization favored at this temperature ? Thermodynamically speaking, explain/support your answer. Show work.PCl5 is known but PI5 is not known Why?
- 1. It measures how good chiral molecules are able to rotate plane, polarized light. A. Specific rotation B. Index of refraction C. Optical activity D. Specific gravity 2. What is the Rf value of the sample if the distance travelled by the solute is 93.5mm and the distance travelled by the solvent is 99.2mm? A. 0.94 mm B. 1.06 C. 0.94 D. 1.06 mm 3. LAL test results could be determined based on ________? I.Color development II. Cloudiness III. Gel clot formation A. I and II B. II and III C. I and III D. I, II, and IIJ 4. Thiamine is assayed by? I. Spectrophotometry II. Fluorometry III. Turbidimetry A. I, II, and III B. I only C. II only D. I and II only4- [PtCl4]? has a center of symmetry, but [NiCl4]? does not. Suggest a correct shape for each of them and draw it.Write the name of the following compounds. Which of them are stereoisomers? Which of them are optically active? Use cis-trans, (E)(Z) nomenclature and assign the absolute configuration (R),(S), if any to each chiral center.