What is the significance of the fact that human mRNA can be accurately translated in E. coli ?

Q: How and why are eukaryotic mRNAs transported and localized to discrete regions of the cell?

A: DNA is the genetic material that houses the genetic information.

Q: What function do the 5' cap and the poly-A tail serve on a eukaryotic mRNA?

A: DNA is a long polymer of deoxyribonucleotides. It is an acidic substance present in the nucleus,…

Q: Why do adult human cells (other than germ cells and stem cells) NOT express the enzyme telomerase?…

A: Telomerase expression is required for cell immortalization ( in cancer cells) and long term tumour…

Q: What is the 5′ cap? How is the 5′ cap added to eukaryotic pre-mRNA? What is the function of the 5′…

A: The process of maturation of eukaryotic mRNA from primary mRNA transcripts is known as RNA…

Q: Two examples utilize RNA reverse transcription in their biology, what are they?

A: Reverse transcription is the exact opposite of the transcription. It involves the synthesis of the…

Q: List three possible uses of site-directed mutagenesis

A: BASIC INFORMATION MUTATION  It is sudden or discontinuous variation These changes occurs in the…

Q: How far apart are transcription bubbles on E. coli genes that are being transcribed at a maximal…

A: The transcription bubble is an area of unpaired bases on one of the uncovered DNA strands. The…

Q: How can the bacteriophage T7 promoter be used to controlexpression of a eukaryotic gene in…

A: Expression vectors are designed to control the expression of particular cloned genes. The native…

Q: What part of the E.coli RNA polymerase specifically binds to the -10 and -35 region of an E.coli…

A: The RNA (ribonucleic acid) polymerase of Escherichia coli has 6 six subunits. The various subunits…

Q: One procedure of obtaining cDNA from mRNA is by using oligo(dT) primers. What are oligo(dT)s? Why…

A: oligo(dT)are  primer widely used for cDNA the synthesis generation of a high frequency of…

Q: What is the mRNA for a Coding Strand of TAT?

A:

Q: How do the mRNAs of bacterial cells and the pre-mRNAs of eukaryotic cells differ? How do the mature…

A: The messenger RNA (mRNA) is a single-stranded RNA molecule in which the genetic information is…

Q: How can a tRNA be engineered to encode for a syntheticamino acid?

A: The standard genetic code features 20 natural amino acids which are the units of proteins. They are…

Q: how can you find genes that are cotranscribed on a single mRNA?

A: Operon model: it had been given by jacob and Monod. this can be only applicable in prokaryotes as in…

Q: What molecular biology strategy can best be used to determine Inhibition of the splicing of one…

A: In eukaryotic genes, introns exist. They are interstitial sequences (80-10,000 bp). They do not code…

Q: How is the poly(A) tail added to pre-mRNA? What is the purpose of the poly(A) tail?

A: Transcription is the first of several steps of DNA based gene expression in which a particular…

Q: How long is the polypeptide produced from the following mRNA transcript?…

A: We can find this answer using a codon chart- 5-UCA UGC UUG GAC UCA AGU CUA CGU GAA U-3' As each mRNA…

Q: Why can it be said that transcription of thebacteriophage T7 genome requires two enzymes?

A: T7 bacteriophage is a small phage which consists of double stranded DNA with 40 kbp nucleotide. The…

Q: What are single-stranded RNA fragments that associate with a protein complex to induce gene…

A: RNA interference, also termed as RNA silencing, is a biological reaction to double-stranded RNA that…

Q: GIVEN THE FOLLOWING DNA SEQUENCES (+) OF THE GENBANK: INDICATE WHICH IS THE TEMPLATE MOLECULE, WHICH…

A: The template strand extends from 3' to 5'. The coding strand, that extends from 5' to 3' in…

Q: What are the steps involved in the processing of eukaryotic pre-MRNA? why is this process important?

A: Eukaryotic pre-mRNA undergoes extensive processing where is pre - tRNA, pre rRNA in both prokaryotes…

Q: What features do mature eukaryotic mRNA molecules have that bacterial mRNAs lack?

A: The process associated with the formation of mRNA (messenger ribonucleic acid) from DNA…

Q: If a gene sequence contains two poly-A sites, the first one in the middle of the sequence and…

A: Introduction: DNA contain the genetic information that transfer from one generation to another. The…

Q: An insertion sequence contains a large deletion in its transposase gene. Under what circumstances…

A: The insertion sequence is the simple transposon of bacteria. Transposons are the DNA segments that…

Q: Know the name of the RNA molecule that is the initial product of the transcription of a eukaryotic…

A:

Q: What would be the consequences if a mutation removed the rut site from this RNA molecule?

A: Mutation is an abrupt change in the DNA sequence and nucleotide base pairs.  Mutation is caused by…

Q: Name and describe three types of modification that the mRNA undergoes in eukaryotes before the MRNA…

A:

Q: Explain how eukaryotic mRNAs are modified to have a cap and a tail.

A: Biomolecules are the compounds that are necessary for different biological processes occurring in a…

Q: In bacteria, single polycistronic MRNA encodes for:

A: This question is based on polycistron.

Q: What is the evidence that some mRNAs are directed to accumulate in specific subcellular locations?

A: mRNA is te transcribed product of the DNA and has an important role in defining the genetic…

Q: What are the three modifications made to pre-mRNA molecules before they become mature mRNAs that are…

A: The unmodified mRNA which is present in the nucleus is known as a pre-mRNA. In eukaryotic cells, the…

Q: What is the meaning of the term consensus sequence? Give an example. Describe the locations of…

A: The consensus sequences are widely used in molecular biology. The genes are controlled by the…

Q: What are the three primary regions of mRNA sequences in bacterial cells?

A: DNA carries the genetic information for the protein production in the body. DNA decoded into mRNA,…

Q: What would be the phenotype of an E. coli strain in which the tandem tryptophan codons in the leader…

A: The set of genes controlled by a single promoter or regulatory sequence associated with that…

Q: What are some key differences in how mRNA is processedin prokaryotes versus eukaryotes?

A: In order to produce a functional protein, the genetic information in the DNA is first transcribed or…

Q: How Can a Sequence Logo Be Used to Identify RibosomeBinding Sites on Bacterial mRNAs?

A: Introduction  Bioinformatic is the advanced and recent branch of biology which utilizes computers…

Q: How are specific mRNAs targeted for decay by deadenylation-dependent or -independent decay?

A: mRNA degradation is a mechanism that eliminates mRNA that is no longer needed in the cell or has…

Q: How Different mRNAs can be produced from thesame primary transcript?

A: The central dogma of biology explains the flow of information from genes to protein by two…

Q: Why aren’t primary rRNA transcripts present in wild-type E. coli?

A: E.coli is a gram negative, faculatative anaerobic, rod-shaped, coliform bacterium of the genus…

Q: What is the purpose of the random hexamer primers?

A: A primer is a nucleotide sequence which acts as a start point for DNA synthesis Primers is…

Q: explain which sequences within the pre-mRNA determine where splicing occurs? How? Why?

A: Splicing of a pre-mRNA molecule occurs in several steps that are catalyzed by small nuclear…

Q: Which eukaryotic RNA polymerase (RNA pol I,pol II, or pol III) transcribes which genes?a. tRNAsb.…

A: RNA polymerase is an enzyme that synthesizes RNA from a DNA template. Different RNA polymerases…

Q: In eukaryotic cells, a poly(A) tail is normally added to pre-mRNA, but not to rRNA or tRNA. With the…

A: Mature RNA molecule results from RNA processing which involves 5' capping, splicing, and addition of…

Q: What are the two important sequence elements in the prokaryotic gene promoter? How does the RNA…

A: PROMOTER:  Promoter sequences are DNA sequences that define the start of RNA polymerase…

Q: Different forms of a protein are produced in the liver and in the brain by alternate splicing of its…

A: Specific mRNAs corresponding to a gene of interest must be identified and reverse transcribed to…

Q: Does RNA Polymerase II terminate transcription at the polyadenylation signal?

A: Since There are multiple questions in the question, I'll answer the first one for you. If the…

Q: What are the steps involved in the processing of eukaryotic pre-mRNA? Why is this process important?

A: In humans or in eukaryotes, the newly made RNA transcript is not yet ready to go and is called as…

Q: Give an account of post transcriptional modification of a eukaryotic Mrna?

A: RNA stands for ribonucleic acid and it is a type of nucleic acid. Some of its important function…

Q: Discuss the advantages, in terms of protein structure and evolution, that result from alternative…

A: Alternate splicing allows a single gene to code for multiple proteins. The protein translated from…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.