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what kind of experiment can prove that azithromycin (antibiotics) has has an effect of on Quorum Sensing in Pseudomonas aeruginiosa.
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- How would someone design an experiment to test the role of azithromycin on quorum sensing in Pseudomonas aeruginosa?Why might bacteria use quorum sensing to regulate genes needed for virulence? How might this reason be related to the rationale behind using quorum sensing to establish a symbiotic relationship?What is the benefit of using LB (Lysogeny broth) medium for the growth of E. coli, Bacillus subtilis and Pseudomonas putida? Why ?
- There are plenty of different phages that will kill Pseudomonas aeruginosa bacteria. Why did the Turner group choose OMKO1?Briefly explain, using the bacteria's response to environment, why the time =0 minutes is often not usable as the first point in log phase.Erythromycin, an antibiotic discovered in 1952 from soil bacterium Saccharopolyspora erythraea, was discovered by a Filipino scientist Abelardo Aguilar. Using schematic diagram, outline the process that led to this
- explain:The process by which alteration in the genetic makeup of live type IIR bacteria was performed by heat killed type IIIS bacteria.What kind of genetic evidence, in addition to 16S rRNA sequences, might have demonstrated that Negativicutes should be considered Gram positive?Why is the fact that Pseudomonas species can grow in nutrient-poorenvironments medically important?
- How long does it take for E. coli to go from lag phase at time 0 to log phase to stationary phase to death phase? Can you point me to a citable source of this time period? I can't find anything online. Thank you.In a rapidly changing environment, which bacterial population would likely be more successful: one that has individuals capable of conjugation or one that does not? Explain.Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage? Show your calculations and give your answer in pfu/ml (pfu = plaque-forming units)