Which are true for paper chromatography? Select the correct response(s): the less polar component will be the last to be eluted the stationary phase is water the less polar component will be the first to reach the solvent front the more polar component will have larger Rf value it can be classified under planar chromatography the chromatographic paper is the stationary phase the more polar component will be the first to be eluted
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- Gas chromatography cannot analyze mixtures due to the sensitivity of the instrument. True FalseChemistry 1. In anion exchange chromatography, the stationary phase negatively charged. a. True b. False 2. Give two (2) examples of a planar chromatographic technique:.Which is correct? Random errors affect accuracy and systematic errors affect precision. An accurate result shows agreement with the true or accepted value. Precise results can contain significant random error, but little systematic error. The standard deviation is a good measure of accuracy. Polydimethylsiloxane (PDMS) stationary phases have low polarities. Which of the following statements is TRUE about gas chromatography using PDMS stationary phases? The gas chromatography column is typically packed with silica microparticles. An oven is used to control the column temperature because a constant temperature is always needed in gas chromatography Octane (C8H18, boiling point: 126 ºC) is eluted later than dodecane (C12H26, boiling point: 216 ºC) 3-Pentanone ((C2H5)2CO, boiling point: 102 ºC) is eluted later than propanol (CH3CH2CH2OH, boiling point: 97 ºC)
- 4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.5) Given the data of the diet sodas, which one would you recommend to be the healthiest? (You may need to research the functions of caffeine, aspartame and benzoate. Please add references if necessary.)1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?Waht is the composition of the reverse phase chromatography? non-polar stationary phase - non polar mobile phase polar stationary phase - non polar mobile phase polar stationary phase - polar mobile phase non- polar stationary phase - polar mobile phase
- You run the chromatography system with paper as the stationary phase and 70% isopropanol as the mobile phase. You determine the Rf of a polar analyte to be 0.40. If you use 100% isopropanol (a less polar solvent) instead of isopropanol as the mobile phase what would you expect to happen to the Rf value? No value effect on Rf higher lower could be higher or lowerExplain the following (1) Column Chromatography(CC) (2) Paper Chromatography (3) Thin Layer Chromatography(TLC) (4) Gas Chromatography (GC)K 32 Uses a lamp and a flame for determining the concentrations of metal ions in solutions. a. High performance liquid chromatography b. gas chromatography c. mass spectrometry d. infrared spectroscopy e. UV/VIS spectroscopy f. atomic absorption spectroscopy g. thin layer chromatography
- Explain the following Chromatography (for drug analysis) Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC)Q.No.3 What is chromatography?. Why chromatography is considered as an important tool for analysis of organic compounds.Classify the descriptions based on whether they apply to ion‑exchange, size‑exclusion, or both types of chromatography columns. - Proteins move through the column at rates determined by their net charge. - The gel bed will contain polymer beads with charged functional groups. - Proteins move through the column at rates determined by their size. - The gel bed will contain porous polymer beads. - The properties of the proteins affect the rate at which they migrate. - Proteins are separated based on differences in their amino acid sequences.