Which of the following conditions would be best to get an enzyme with a pl of 7.0 to stick to a cation exchange column as part of the purification protocol so that the resulting pure enzyme can be used in kinetics studies? High salt concentration and a pH-6.0. High salt and a pH-7.5. Protein has a pl of 7.0 and a pH-7.0. Low salt concentration and a pH-6.5. Protein has a pl of 7.0 and a pH-14.

Which of the following conditions would be best to get an enzyme with a pl of 7.0 to stick to a cation exchange column as part of the purification protocol so that the resulting pure enzyme can be used in kinetics studies? High salt concentration and a pH-6.0. High salt and a pH-7.5. Protein has a pl of 7.0 and a pH-7.0. Low salt concentration and a pH-6.5. Protein has a pl of 7.0 and a pH-14.

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter23: Enzymes

Section: Chapter Questions

Problem 23.6P

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