Which of the following is a unique attribute of CRISPR/Cas9 compared to restriction enzymes Group of answer choices it can be targeted to any specific region of the genome as long as there is an adjacent PAM motif it was first discovered in bacterial cells it can cut double-stranded DNA sequences it can be used for molecular cloning experiments

Which of the following is a unique attribute of CRISPR/Cas9 compared to restriction enzymes Group of answer choices it can be targeted to any specific region of the genome as long as there is an adjacent PAM motif it was first discovered in bacterial cells it can cut double-stranded DNA sequences it can be used for molecular cloning experiments

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 5QP

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Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5
What are "genetic biohackers" in reference to "do it yourself CRISPR kits"? Who is Josiah Zayner and what is he infamous for? Feel free to search that online...
Which of the following is NOT a true statement about CRISPR/Cas9?A. CRISPR/Cas9 is simpler to design than other gene editing technologies.B. CRISPR/Cas9 is derived from a bacterial adaptive immune defense system.C. CRISPR/Cas9 is more efficient than other gene editing technologies.D. CRISPR/Cas9 uses an RNA guide to edit a gene.E. Delivery of CRISPR/Cas9 is easier than delivery of other gene editing technologies.
1. MEGA works differently than traditional CRISPR technology by targeting mRNA and NOT DNA sequences 2. MEGA is more specific and safer than traditional CRISPR technology, making it a novel and useful tool for gene editing purposes 3. MEGA may be suited as a possible strategy to enhance gene therapy approaches for patients with genetic disease such as blood cancers
The genomic DNA of a bacterial cell is not destroyed by the cell’s own restriction enzymes because choose 1 answer below: the bacterial DNA is too small to contain the recognition sequence for the enzymes. the restriction sites are occupied by histones. the genome is protected by the nuclear membrane. the pH in the bacterial cell does not allow the restriction enzymes to function. None of the above
Fluorescently-labeled dideoxynucleotides are used in _____. Group of answer choices:" cDNA libraries DNA sequencing genomic DNA libraries PCR
CRISPR RNA (crRNA) in a bacterial cell: is a snippet of genetic material from a virus stored in a bacterial chromosome. is clipped from an invading virus. is used to identify and destroy infecting viruses. guides Restriction Enzymes to specific sites to carve viral DNA into fragments.
You need to complete the following steps for an experiment. Explain whether an acrylamide gel or an agarose gel is more appropriate to use for the following experiments. Purifying tRNA from total RNA Isolating a vector insert for cloning Analyzing PCR products
Which of the following is NOT a tool used in recombinant DNA technology? Cutting specific DNA sequences using restriction enzymes. Adding Taq polymerase to copy DNA in PCR. Using primase to catalyze the formation of phosphodiester bonds. Inserting a gene of interest into a vector. Inserting a plasmid into bacteria to generate copies.
In biotechnology procedures, a(n) ____ is a nucleic acid fragment that is used to search for and identify a sequence of interest. vector probe library antibody plasmid
The other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double stranded
Which of the enzymes from the following list wouldyou need to make a cDNA library? What is the function of those enzyme(s) in the process?a. DNA polymeraseb. RNA polymerasec. A restriction enzymed. DNA ligasee. An aminoacyl-tRNA synthetasef. Peptidyl transferaseg. Reverse transcriptase