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- which of the following statements is incorrect? A. a conventional mass spectrometer does not use a spectrophotometric detectorB. a conventional mass spectrometer does not always require high purity samplesC. a mass spectrum shows no signals due to uncharged speciesD. a conventional mass spectrometer uses high energy UV radiationWhich part of the instrument perform the given function introduction of a micro amount of sample converts sample components into gaseous ions orts the ions according to m/z converts m/z values to electrical signals converts signal/results into a mass spectrum A. mass analyzer B. Vacuum system C. Ion source D. Processor E. inlet system F. detectorsThe validation process comprises four main steps that are : hardware validation, software validation, method validation and system suitability. To evaluate a spectrophotometer’s accuracy by measuring absorbance, a solution of 60.06 mg/L K2Cr2O7 in 0.0050M H2SO4 was carefully prepared together with a 0.0050 M H2SO4 as a reagent blank. If the spectrometer’s signal is properly calibrated, it has been reported that this solution should provide an absorbance of 0.640 +- 0.010 at wavelength 350 nm. Which one of the validations mentioned above had been involved in this process? Explain your choice
- Where can I know the spectral properties of my sample? UV vis spectro, fluorescence spectro, Portable NIRTo determine the content of Pb in a canned sausage, 5.00 g were weighed and after adequate treatment they were gauged to a volume of 100 mL, from there an aliquot of 2 mL was taken and brought to a volume of 250 mL. The readings of a series and of the sample were taken in an Atomic Absorption spectrophotometer, recording the following data: mg Pb/mL 0 1 2 3 4 Sample Absorbance 0.00 0.15 0.29 0.44 0.58 0.38 What is the% Pb in canned sausage?1. An unknown sample of Cu2+ gave an absorbance of 0.262 in an atomic absorption analysis. Then 1.00 mL of solution containing 100.0 ppm Cu2+ was mixed with 95.0 mL of unknown, and the mixture was diluted to 100.0 mL in a volumetric flask. The absorbance of the new solution was 0.500. a) Denoting the initial unknown concentration as [Cu2+]i, write an expression for the final concentration [Cu2+]f, after dilution. Units of concentration are ppm. b) In a similar manner, write the final concentration of added standard Cu2+ designated as [S]f. c.) Find the [Cu2+]I in the unknown
- how the principle of spectrophotometric experiments? Explain plz thank youA solution with a known concentration equal to 3.4 mM gave an absorbance reading of 0.820 in a spectrophotometer. Assuming that there isa linear relationship between concentration and absorbance up to a concentration of 4.0 mM, what would the absorbance reading be for asolution with a concentration 1.5 mM?If a TOF mass spectrometer needs to scan an m/z of 600, what is the speed of this ion if it is accelerated through 4000 volts? How long will this ion (at the speed calculated) take to drift 2.00 m through the spectrometer? How many mass spectra can be taken each second if a new cycle begins each time this ion reaches the detector?
- . A sample of cobalt is to be determined using absorption spectrum analysis. A 0.286 g sampleof a cobalt ore is crushed and digested in 1M HNO3. The stock solution is then diluted to 250.0mL in a volumetric flask (assuming the solution has a density = 1.00 g/mL). The analysis thenproceeds as follows: Sample Solution Std Co solution (11.2 ppm) Final Volume Transmittance 15.0 mL 0.00 mL 100.0 mL 54.1% 15.0 mL 5.00 mL 100.0 mL 19.9%Calculate the percent cobalt in the original ore sample.A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM)…A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM)…
