Which of the following is TRUE about the Nitrate Reduction test? Question 1 options: A Nitrate oxidase is the enzyme present for a positive test result B Pseudomonas aerugenosa can use oxygen instead of NO2 for aerobic respiration C Ammonia is reduced to NO2 by some bacteria D Oxygen is needed to reduce nitrate E It tests the ability to reduce NO3 to NO2 or ammonia or nitrogen

Which of the following is TRUE about the Nitrate Reduction test? Question 1 options: A Nitrate oxidase is the enzyme present for a positive test result B Pseudomonas aerugenosa can use oxygen instead of NO2 for aerobic respiration C Ammonia is reduced to NO2 by some bacteria D Oxygen is needed to reduce nitrate E It tests the ability to reduce NO3 to NO2 or ammonia or nitrogen

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.8: Fecal Occult Blood Test

Problem 1CS

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Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive test
Which of the following is FALSE? Question 3 options: A Some bacteria reduce nitrate to nitrite B Some bacteria reduce nitrite to ammonia and/ or nitrogen gas C Sukfanilic acid and alpha- naphthylamine are used to detect if nitrites are present D A clear result after adding reagent A and B is negative for nitrate reduction E A clear result after adding zinc is a positive result for nitrate reduction
The number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar by the pour plate method. The plates were inoculated aerobically for 48 hours at 37°C. What can you conclude from this data? Why? Mouthwash CFU per ml in salvia Before Use After Use Listerine 2.22 x 107 1.22x107 Schmidts Mouthwash 2.42 x 107 3.22x105 Biotene Mouthwash 3.21 x 107 2.12 x106
2.1The number of bacteria in the samples need to be quantified. The laboratory uses a specificprotocol where the dilution factor on the spread plates must be:10-6; 10-7; 10-8Describe how you would prepare the required dilution series using the least amount of dilutionwater. 2.2 Calculate the amount of bacteria in the sample from the following results (show the steps in the calculation): Dilution factor Count10-6 27110-7 3510-8 21
This test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours. Task: In a tabulated form, determine if the Escherichia coli are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.
Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained cells.Consult your chart at the beginning of this lab report if you need help remembering the correct Gramreaction for each species. Choices may be used more than once. Staphylococcus aureus after decolorization
You are asked to prepare Murashige & Skoog (MS) medium according to concentration shown the Table. Answer the following question: 1. Prepare 250 mL stock solution (200X more concentrated) of ammonium nitrate and KNO3. 2. From the prepared ammonium nitrate stock solution above, calculate the volume required to prepare 200 mL MS medium.
Why are the Methyl Red/Voges-Proskauer tests done after 48 hours of incubation?a) Readings taken at earlier time points may be different from readings taken at 48 hours b) Bacteria need 48 hours to process all the oxygen in the media c) Bacteria grow slowly in the MR/VP media d) K2HPO4 needs 48 hours to react with the acids produced by fermenting bacteria
I. Match the bacterium being described in column X with its best classification found in column Y. Choose the best answer. Column X1. Staphylococcus aureus can be grown on mannitol salt agar that contains 7.5% NaCl. The bacterium is________. 2. Lactobacillus acidophilus grows best at pH 4.5. It is considered a(n) ________. Column Y A. acidophile B. alkalophile C. barophile 3. A bacterium that thrives in the Great Salt Lake but not in fresh water is probably a ________.4. Bacteria isolated from the bottom of the ocean need high atmospheric pressures to survive. They are________.5. A bacterium that thrives in a soda lake where the average pH is 10.5 can be classified as a(n) ________. D. halotolerant E. halophile
what does a negative result mean in an oxidase test?Select all that applies a)The bacteria may undergo anaerobic respiration b)The bacteria may have a cytochrome C oxidase c)The bacteria may undergo aerobic respiration d)The bacteria will not have cytochrome C oxidase e)None of the anwers are correct
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: In a tabulated form, determine if Salmonella typhi the are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: In a tabulated form, determine if the Pseudomonas aeruginosa, are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.

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A	Nitrate oxidase is the enzyme present for a positive test result
B	Pseudomonas aerugenosa can use oxygen instead of NO2 for aerobic respiration
C	Ammonia is reduced to NO2 by some bacteria
D	Oxygen is needed to reduce nitrate
E	It tests the ability to reduce NO3 to NO2 or ammonia or nitrogen