困Topic: laboratory InstrumentWhich of the following islare TRUEWhich of the following is/are TRUEregarding microscopes? *regarding the use of laboratory glass waresand instruments? *TEM gives excellent view of internalstructures of a specimen in three-dimensional imageBoth the inoculating loop and inoculatingneedle can be used in nutrient broth.Phase contrast microscope permitsA serologic pipette has a bulbous partwhich is used to deliver different volumesdetailed examination of internal structuresof live specimensof liquid.A glass slide and coverslip are to observeInterference microscope creates twostained microorganisms.superimposed images of a specimen.7:14 pmP Type here to searchヘロG) ENG30/09/2021近

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Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter3: The Microbiology Laboratory

Section: Chapter Questions

Problem 3TBP

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discuss the microscopy technique that gives the best constrast image of non biological samples such as micro and nano particles?
Student Y is working with his microbiology experiment, the directions of the agar is to suspend 25g in 1000mL of distilled water, heat to boiling until completely dissolve. How much of the agar does he need to prepare for 18 petri dishes?
Discuss the microscopy technique that gives the best contrast image of non-biological samples such as micro and nano-particles.
a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.
Describe the operating features of electron microscopes andhow they differ from optical microscopes in illumination source,magnification, resolution, and image appearance.
discuss the use and applications of MICROSCOPE in microbiology and clinical studies. Parts and functions. Cite application of microscopy in the discussion
Explain in detail the study of microbial structure microscopy.
Compare the appearance of microorganisms you observed using phase-contrast microscopy versus brightfield microscopy.
Which microscopy technique is BEST at determining the presence of physical microbial interaction(s)? a Light microscopy. b Scanning or transmission electron microscopy. c Fluorescent microscopy. d Phase contrast microscopy.
1. Differentiate the compound light microscope from the dissecting microscope in terms of: *image produced *magnification *type of specimen examined 2. Write down the principles of the following: *electron microscopy *scanning tunneling microscopy *atomic force microscopy
Differentiate Brownian movement from true motility Methods: Place the prepared specimen under the microscope. Observe for Brownian movement (Non-motile organisms are tiny particles in suspension. Also, they exhibit movement characterized by an oscillating, quivering motion.) Or and True motility (Motility of microorganisms is an independent, true movement of translation brought about by organs of locomotion or by gliding.) With the presented specimen, observe and determine whether the specimen shows true motility or brownian movement.
EXPERIMENT 2 Title: Aseptic technique Objectives: To apply the aseptic technique To observe the growth found on the petri dish. Material/apparatus: Gloves, incubator, soap for handwashing, tissues, nutrient agar, 70% alcohol Procedure: Wipe off the bench with 70% alcohol. Draw a line down the middle of the petri dish to divide the plate in half. Label each halves with A and B. Press your unwashed thumb onto the agar at column A. Apply proper handwashing technique. Put the same thumb after washing onto agar at the column B. Incubate the petri dish at 37°C for 24 hours or overnight. Observe and note down the colonies. Results:

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