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- Suppose you were instructed to make two antiserum solutions: The first is identical to what you used in the lab exercise. The other is a 10:6 dilution of the antiserum. After incubation with the antigen, the full-strength antiserum produces a precipitous ring, but the diluted antiserum does not. Explain these results. Is this due to poor specificity or sensitivity of the test?To determine whether an enzyme-linked antibody is bound to an antigen, __________ must be added, which results in a color change. the enzyme’s substrate alkaline phosphatase a buffer an antibody an enzymeWhich of the following statements regarding sandwich ELISA is correct? Select one: A. The detection antibody can bind to the Fc region of the capture antibody. B. Antigens are sandwiched between the detection and the enzyme-linked antibodies. C. Washing steps are needed after addition of an antibody. D. The capture and detection antibodies recognize the same epitope on a captured antigen. E. Antigens are coated on the surface of a microtiter plate.
- The disc diffusion assay measuring a disinfectant's effectiveness against a microorganism is more standardized than measuring an antibiotic's effectiveness with the kirby-Bauer assay. a) True b) FalseTrue or false 1. Heterogenous antibodies used for structural studies can be obtained by monoclonal hybridoma technique. 2. PrP(Sc) is largely alpha-helical structure.Increasing the tube voltage on a clinical x-ray unit: a. Improves the resolution of an x-ray image. b. Increases the intensity of the Characteristic x-rays. c. Increases the intensity of the Bremsstrahlung x-rays. d. Increases the energy of the Bremsstrahlung x-rays. e. Increases the energy of the Characteristic x-rays. and why??
- Choose the combination of answers that most accurately completes the statement. Which statement is not an aim in the use of drugs in antimicrobial chemotherapy? The drug should a. have selective toxicity b. be active even in high dilutions c. be broken down and excreted rapidly d. be microbicidalHow can you use Uv-visible spectroscopy in qualitative and quantitive analysis of drugs? Please answer shorty at your own words. Answer should be to the point(specific 4-5 lines)The Fab regions of an antibody are made of which protein chain? A) a heavy chain containing only constant regions B) a light chain containing variable and constant regions C) a heavy chain containing variable and constant regions D) both a light chain and a heavy chain containing variable and constant regions E) both a light chain containing variable and constant regions and a heavy chain containing only constant regions
- Which of the following is not a property of an antimicrobialagent?(a) Must have a favorable chemotherapeutic index(b) Must be selectively toxic(c) Must be effective against viral diseases(d) Must have a known concentration over a period oftime required to eliminate a pathogen(e) b and cE-strip testing can be used to determine a drug MIC against an organism. Which ONE of the following is correct? Select one: A. The zone of inhibition is inversely proportional to the activity of the drug B. The E-strip result guides to the maximum drug concentration that can be used to clear an infection C. The E-strip is impregnated with a fixed concentration of drug D. The E-strip is used broadly clinically to determine if an organism is resistant to a drug E. The MIC is read where bacterial growth touches the E-stripA factor in the zone of inhibition size on the Kirby-Bauer plate is: a. amount of medium placed on the plate b. rate of diffusion of the drug used c. stage of growth of the microbe placed on the plate d. all of these