To determine whether an enzyme-linked antibody is bound to an antigen, __________ must be added, which results in a color change.   the enzyme’s substrate alkaline phosphatase a buffer an antibody an enzyme

Q: Which of the following statements is true? O Streptolysin-O is a growth factor for white blood…

A: ASO is test which is used to measure the anti o antibodies titre in patients serum.

Q: Antisera used in blood typing contain:        A. both agglutinins and agglutinogens B. agglutinins…

A: Blood typing is a test performed to determine the blood group. It is also known as ABO typing.  In…

Q: n western blotting, secondary antibody is conjugated with color or fluorescence producing enzyme…

A: In recombinant DNA technology, several group of techniques are involved. These techniques are…

Q: What is the purpose of using a secondary antibody?

A: An antibody is a protein structure raised against a specific antigen. The antibody is denoted as Ab…

Q: I need to make an antibody for the extracellular portion of the Notch receptor. I have made S35…

A: Hybridoma technology is used to produce a large number of monoclonal antibodies. Monoclonal…

Q: Which protein of the following has a catalytic role? Antibody Enzyme Collagen Hormone

A: Catalyst: These are the substance that increases the chemical reaction. In the process, these…

Q: Positive sample negative sample 5. Unbound labeled antibody is washed away and a colorimetric…

A: ELISA - Enzyme linked immuno sorbant assay  The ELISA technique is used to detect the presence of…

Q: An antibody consists of four protein chains, two______chains and two_______ chains. Each is composed…

A: The molecules bind to one of the two types of protein receptors of the immune system. One of the…

Q: What is ion-exchange chromatography based on? moving a molecule with a net charge in an electric…

A: Ion exchange means the exchange of ions with protein molecules. In chromatography, there is a…

Q: Several classes of LDLreceptor mutations have been identified as causes of this disease. Suppose…

A: Any alternation in the sequence of nucleotide in the genome of any organism is known as mutation.…

Q: Two antibodies, one is IgM and the other is IgG, both can bind to the same epitope. Which of the…

A: IgM and IgG are two very important antibodies. IgM is the first antibody to appear in response to…

Q: In which of the following cases would treating a patient with an antiserum (purified antibodies) be…

A: Antiserum  Antiserum is extracted from the animals blood like rabbit or horse to treat specific…

Q: Based on these data, which of the following proteins (biotin, hygromycin, and streptavidin) can rule…

A:

Q: Calculate the rough cost estimation of manufacturing 250g of antibodies in 50 L bioreactor using CHO…

A: The biological function of antibodies is to bind to pathogens and their products, and to facilitate…

Q: When an enzyme-linked antibody is added in an ELISA test (select all appropriate),

A: ELISA or Enzyme-Linked Immunosorbent Assay is an effective method by which antibody present in the…

Q: Refer to the illustration above. The most likely reason for Response- Il being greater than Response…

A: An immune response is an organism's reaction to protect itself from external invaders such as…

Q: n agglutination reactions, the antigen is a……… in precipitation reactions, the antigen is a……………

A: The particles clump or agglutinate when a particulate antigen is combined with its antibody in the…

Q: A) What  additional reagents would you need to perform immunostaining of frozen sections of mouse…

A: The process used here is immunohistochemistry followed by immunostaining. Immunohistochemical…

Q: Which

A: Introduction :- The sandwich enzyme linked immunosorbent assay(ELISA) is a Sensitive and robust…

Q: How does horseradish peroxidase (HRP) contribute to creating the final colored sample? O A. HRP is a…

A: In biochemistry techniques, the enzyme horseradish peroxidase (HRP), which is isolated from the…

Q: N-glycosyltransferase attaches which sugar to the base oligosaccharide to synthesize the B antigen?…

A: The antigens A, A1, B, and AB, which are expressed differently on the surface of RBCs, other cells,…

Q: Please outline the general steps you would take to test your hypothesis that SIN-47 works by…

A: Hypothesis: SIN-47 works by disrupting the interaction between LEK2 and JAK4.

Q: Certain antibodies bind solely to the folded structure of their target protein ligand, whereas…

A: The innate and adaptive immunity: The antibody possesses two structural components called paratopes…

Q: booster antigen 1 month after the initial inoculation, and collect antibodies (antiserum) 1 week…

A: Having considered how an appropriate primary immune response is mounted to pathogens in both the…

Q: The kit for the homogeneous assay for methamphetamine comes with methamphetamine linked to…

A: Methamphetamine is a class of phenethylamine drugs which has psychoactive effects imitates the…

Q: Suppose you were instructed to make two antiserum solutions: The first is identical to what you used…

A: Precipitation test is a antigen and antibody reaction in a given test solution to form a insoluble…

Q: The figure below shows the structures of the blood group antigens O, A and B.List all nucleotide…

A: The ABO blood group is an example of multiple alleles in which total three alleles are responsible…

Q: Which of the following red cells will react strongest with anti-H? O a. A2 red cells O b. O red…

A: H antigen is present on RBCs. It acts as precursor for ABO blood group system. Modification of H…

Q: Differentiate between the serological tests used to identify isolatedcultures of pathogens and those…

A: Serologic tests are blood tests done for antibodies in the blood. They can involve a number of…

Q: You perform an ELISA to test hospital soap samples for contamination with Pseudomonas aeruginosa.…

A: ELISA or enzyme-linked immunosorbent assay is a Molecular Biology technique used to detect and…

Q: I want clear handwritten solution only....i will up vote

A: Biological recognition elements are described as the biological molecules that interact specifically…

Q: Labs that do a lot of Western blots often have hundreds of primary bodies but only two or three…

A: Western blotting involves binding of a primary antibody to the target antigen followed by binding of…

Q: Digestion of antibodies with mercaptoethanol yields: Select one: 2 Fab,  Fc 2 Fab, and 1 Fc 4…

A: Antibodies are polypeptide chains produced in the body in response to an antigenic attack, also…

Q: Would the same procedure done in Minimum Inhibitory Concentration (MIC) be applicable in determining…

A: Answer- Minimum inhibitory concentration (MIC) is the value of an enzyme or a drug which is required…

Q: Which of the following is TRUE about immunofluorescence microscopy where we localize proteins using…

A: * Immunoflurescence microscopy is used to light microscopy with a fluorescence microscope which is…

Q: O Antibody interference occurs when (multiple possible answers) Choose all that apply. O free…

A: In immunoassays, the interaction between the contents in the sample with one or more antibodies is…

Q: Which of the following are elements of the structure of antibodies? Select all that apply.       The…

A: Antibody , also known as immunoglobulin, functions in immune defense by neutralizing the antigens.…

Q: In general, protein detection methods are based on:   a. fluorescence b. column affinity c.…

A: Protein It is one of the biomolecule which is made up of amino acids. In a protein, amino acid…

Q: hoose all that are correct about indirect ELISA. A) An antigen can bind the substrate B) A…

A: ELISA is also called as the enzyme linked immunosorbent assay. It is an assay technique. This…

Q: Below is shown the FRAP profiles of three proteins X, Y and Z, which of the protein(s) is/are most…

A: FRAP stands for fluoresence recovery after photobeaching(FRAP) is a technique to study mobility of…

Q: In determing Rh group, do you consider the control tube for reading when it shows an agglutination…

A: The system of the Rh blood group is used to classify the blood groups depending on the existence or…

Q: Certain naphthalene derivatives, such as the dansyl group, exhibit a weak yellow fluorescence when…

A: Dansyl chloride is a reagent that reacts with primary amino groups in both aliphatic and aromatic…

Q: Explain this assertion using the example of hematoxylin and eosin staining.

A: Hematoxylin and eosin staining is used for identification of diseases like cancer. These are two…

Q: Why is it important to measure the pharmacokinetics(PK) and metabolism of an antibody drug

A: Pharmacokinetics studies the changes of drug concentration in the body and the total amount of drug…

Q: A calibration curve is prepared from a series of antibody concentrations from 0.5 mg/dL to 3 mg/dL.…

A: The use of calibration curve and the calibration curve equation is one of most widely accepted…

Q: A synthetic immunoglobulin (Protein X) was created by a scientist to recognize retroviruses.…

A: SDS is a very common detergent used to denature and reduce protein. A protein X will not be…

Q: The valence of an antibody that is a pentamer is: 1) 2 O 2) 4 3) 3 4) 10

A: The higher an antibody valency which is the number of antigen binding sites, the higher the amount…

Q: What chemical ingredient makes MSA selective? This chemical ingredient makes MSA selective because:

A: Note:Please note that we cannot complete the table since it is experimentally based and as per our…

Q: A thyroid peroxidase antibody test was performed on a 45 year old man. The dilution sequence was 25…

A: Dilution is the process of decreasing the concentration of a solute in a solution, usually simply by…

Immunology

Immunology is the division of science involved with the immune reactions occurring in living organisms. The organisms are accustomed to evolving internal defense mechanisms to evade the invasion of microbial pathogens. Diverse cells are connected with stimulating the immune responses, which produce inhospitable conditions to the microbes prompting their lysis and thus inhibits the multiplication of the pathogens. The immune system can distinguish the pathogens through the aid of cellular intercommunication and launch responses to destroy them.