Why did you measure 60 ml and not 59.04 ml to make up this final solution?

Macroscale and Microscale Organic Experiments
7th Edition
ISBN:9781305577190
Author:Kenneth L. Williamson, Katherine M. Masters
Publisher:Kenneth L. Williamson, Katherine M. Masters
Chapter7: Extraction
Section: Chapter Questions
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Why did you measure 60 ml and not 59.04 ml to make up this final solution?
2.
You want to do an experiment on cells to treat them with very low
concentrations of both Atrazine and DMSO (which is the solvent you need to use to
make your atrazine stock solution). To do this you must first make a relatively
concentrated stock of atrazine (3 x106 ppm Atrazine, DMSO) by dissolving powdered
atrazine in DMSO.
Next you use the (3 x106 ppm Atrazine, DMSO) stock to make a less concentrated solution by
combining:
1 μL of (3 X 106 ppm Atrazine, DMSO)
0.999 ml of cell culture media
To get to your experimental conditions you combine the following 2 liquids (consider the final
volume of this mixture = 60 ml for your calculations).
X83.A
60 μl of the solution you prepared in the highlighted text
60 ml of cells and cell culture media
f. Why did you measure 60 ml and not 59.04 ml to make up this final solution?
Transcribed Image Text:2. You want to do an experiment on cells to treat them with very low concentrations of both Atrazine and DMSO (which is the solvent you need to use to make your atrazine stock solution). To do this you must first make a relatively concentrated stock of atrazine (3 x106 ppm Atrazine, DMSO) by dissolving powdered atrazine in DMSO. Next you use the (3 x106 ppm Atrazine, DMSO) stock to make a less concentrated solution by combining: 1 μL of (3 X 106 ppm Atrazine, DMSO) 0.999 ml of cell culture media To get to your experimental conditions you combine the following 2 liquids (consider the final volume of this mixture = 60 ml for your calculations). X83.A 60 μl of the solution you prepared in the highlighted text 60 ml of cells and cell culture media f. Why did you measure 60 ml and not 59.04 ml to make up this final solution?
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