Why do you have to reduce the amount of light with the diaphragm in order to see bacteria in a hanging drop slide? What is the value of a hanging-drop preparation? Wet-mount preparation?
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- What is the best wavelength to study light scattering with bacteria? 260 nm 260-280 nm 450 nm 280 nmIf we used the 365nm setting on the UV light box would this have caused more or less UV light damage, explain why?In absorption spectrophotometry: a. Absorbance is directly proportional to transmittance b. Percent transmittance is directly proportional to concentration c. Percent transmittance is directly proportional to the light path length d. Absorbance is directly proportional to concentration
- to view bacterial cells and structures clearly using the light mircoscope, what object lens much be used to view the bacteria _______ , and what substance is used to prevent light scattering and light behind with this objective _______?Why do you use immersion oil with 100X objective lens?What is the principle behind a UV-Vis spectrophotometer, and what are its key components, and how does it work?
- Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourcewhat effect would using an old Gram's crystal violet preparartion containing crystallized dye have on your judgment of the gram reaction of an organism?Why do you need to move the lever of the aperture diagram all the way to the left (smallest value) before you start viewing your sample?