Why is a hypotonic buffer used for the resuspension? 1.DCPIP passes easily across the intact chloroplast membrane, but can't get into the thylakoid, so electron transport could not be reliably measured if using a hypotonic buffer. 2.To gently lyse the chloroplasts, leaving the electron transport membranes intact, whilst allowing DCPIP access 3.To gently separate the chloroplasts from each other, leaving the electron transport membranes intact, whilst allowing DCPIP access 4.DCPIP cannot easily across the intact chloroplast membrane, so electron transport could not be reliably measured if using a hypotonic buffer.
Why is a hypotonic buffer used for the resuspension? 1.DCPIP passes easily across the intact chloroplast membrane, but can't get into the thylakoid, so electron transport could not be reliably measured if using a hypotonic buffer. 2.To gently lyse the chloroplasts, leaving the electron transport membranes intact, whilst allowing DCPIP access 3.To gently separate the chloroplasts from each other, leaving the electron transport membranes intact, whilst allowing DCPIP access 4.DCPIP cannot easily across the intact chloroplast membrane, so electron transport could not be reliably measured if using a hypotonic buffer.
Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter9: Photosynthesis: Capturing Light Energy
Section9.4: The Light-dependent Reactions
Problem 6LO: Describe the flow of electrons through photosystems I and II in the noncyclic electron transport...
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Why is a hypotonic buffer used for the resuspension?
1.DCPIP passes easily across the intact chloroplast membrane, but can't get into the thylakoid, so electron transport could not be reliably measured if using a hypotonic buffer.
2.To gently lyse the chloroplasts, leaving the electron transport membranes intact, whilst allowing DCPIP access
3.To gently separate the chloroplasts from each other, leaving the electron transport membranes intact, whilst allowing DCPIP access
4.DCPIP cannot easily across the intact chloroplast membrane, so electron transport could not be reliably measured if using a hypotonic buffer.
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