Q: How do you make liquid bacteria culture?
A: It is important for growing more numbers of bacteria to isolate enough plasmid DNA for experimental…
Q: If the iodine step in a Gram-staining procedure was omitted, what color would you expect…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: Why does a streaking method used to inoculate plates result in isolated colonies?
A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…
Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?
A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…
Q: How does the heaviness of a bacterial smear affect its microscopic analysis?
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: What kind of medium might you make to selectively grow a bacterium that lives in your gut?
A: MacConkey agar is used as a selective and differential media for differentiating gut bacteria which…
Q: Is it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What…
A: There are two main microbiology methods used for isolating bacteria from a clinical sample in order…
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: A Gram stain indicates purple cells that are round and attached in chains. Which of the following…
A: Gram staining is a simple technique, in which two categories of bacteria are identified based on…
Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…
A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…
Q: In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT:…
A: Organisms can be provisionally identified by growing them on medium and looking at their shape and…
Q: Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown…
A: Endospores are dormant, tough, vegetative structure produced by the bacteria from the Firmicute…
Q: What Are The Conditions When Gram Positive Bacteria Can Appear Gram Negative?
A: Gram positive bacteria have thick cell wall and they give positive result in gram stain test , in…
Q: citrobacter freundii
A: Citrobacter freundii is a species of anaerobic gram-negative bacteria which belongs to the family…
Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…
A: Microbiology is the branch of science which deals with the study of different types microorganisms…
Q: You perform a Gram stain. Your Gram positive control, your Gram negative control, and your sample…
A: Gram-staining is a technique used to classify bacterial species, either as gram-positive or…
Q: Describe the bacterial colonies providing information on shape, color, size, elevation and edge…
A: *morphology of Escherichia coli is identified as rough or a smooth form. *The trough forms colonies…
Q: The rapid identification methods used in this activity are designed to identify __________.…
A: Bacteria are microscopic, single-celled, prokaryotic organisms. They are much smaller than other…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: what is the gram staining procedure for e-coli
A: Gram staining is the laboratory technique which is used to differentiate between the morphology of…
Q: what is the culture media for gram-positive bacteria?
A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…
Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…
A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…
Q: Why did we both stab and streak our blood agar plates when inoculating them with bacteria?
A: Blood agar is a nutrient medium which contain essential nutrients for bacterial growth and blood of…
Q: List 2 reasons why a bacterium that should be gram positive might turn out gram negative.
A: Gram-positive and Gram-negative bacteria are differentiated by Gram staining.
Q: Streaking One drop of bacterial solution contains millions of cells. To identify a species,…
A: The purpose of streaking for isolation is to produce isolated colonies of an organism on an agar…
Q: When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal…
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…
A: The gram staining procedure that is used for staining and visualizing the bacteria. The bacteria are…
Q: erial slide agglutination technique important in diagnostic procedure?
A: Immunoassays are bioanalytical ways that use the specificity of an antigen-antibody reaction to…
Q: How should agar plates be incubated? Why?
A: Incubating the plates to stimulate the growth of microbes is a crucial step in any microbiology…
Q: What technology is available for faster bacterial culture?
A: BASIC INFORMATION BACTERIA It is one of the type of biological cell. They belong to prokaryotic…
Q: What color would the bacterial cells have when viewed microscopically after the following treatment?…
A:
Q: What is the shape of bacteria under a microscope, what color is there, and is there Gram staining is…
A: Introduction- Gram staining is basic staining to identify the bacteria of medical significance. Gram…
Q: How does the bacterial cell structure contribute to the mechanism of Gram staining?
A: Bacteria are prokaryote organisms, that lack well-defined cell organeles and nuclei. Bacterial cell…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: What is the definition of Gram Positive?
A: When the stain combines with bacteria in a sample and the bacteria will either stay purple or turn…
Q: What is gram staining Step by step procedure Explain its basis and how it differentiate bacteria use…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: What color would the bacterial cells have when viewed microscopically after the following…
A: Gram staining is a technique proposed by Christian Gram to distinguish Gram positive and negative…
Q: Why can't gram staining be used for mycobacterium?
A: Gram staining is one of the staining techniques. This is usually used for differentiating between…
Q: Examine the image below. The bacteria in this image have been treated with gram straining procedure.…
A: The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring…
Q: What color would the bacterial cells have when viewed microscopically after the following treatment?…
A: Gram staining is a technique to distinguish between two broad types of bacterias on the basis of…
Q: identifying the type of bacteria I have cultured. I know that it is gram-negative and cocci in…
A: There is only two type of bacterias which do not grow so well on EMB medium. 1. Shigella 2.…
Q: Which bacterial type ( Gram positive or Gram negative) is more sensitive to the antibiotic…
A: Penicillin is the group of antibiotics which were originally derived from the penicillium molds. It…
Q: Why does nigrosin not stain the bacteria?
A: Nigrosin is an acidic stain, used in the negative staining technique. Staining is a part of…
Q: What can happen to cause gram-positive bacteria to appear gram-negative?
A: There are numerous methods of characterizing bacteria. One strategy depends on the cell membrane. In…
Q: You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix.…
A: In serial dilutions, dilutions are multiplied at each step. We can calculate dilution by dividing…
Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…
A: Bacteria are the prokaryotes which fall under Kingdom Monera. They can be found everywhere. They…
Q: A microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of…
A: Introduction Helicobacter pylori, formerly known as Campylobacter pylori, is a spiral (helical)…
Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…
- Why is it important to follow sterile technique?
- What can happen to cause gram-positive bacteria to appear gram-negative?
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- Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?What can happen to cause gram-positive bacteria to appear gram-negative?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
- Which bacterial type ( Gram positive or Gram negative) is more sensitive to the antibiotic penicillin? Explain why?Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?Describe one error in the staining procedure that would result in a Gram-positive bacterium appearing pink.