Why do you think the continuous culture has higher chances of contamination than batch and fed-batch culture?

Q: What is a pure culture? Differentiate axenic culture from pure culture

A: Among the living beings on this planet, microbes play a very important role in the ecosystem, apart…

Q: student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step…

A: 5. The loop was flamed

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: Why are the results of plate counts expressed as the number of colony-forming units, rather than the…

A: Microbes are small living organisms  which can not be seen through naked eyes. The study of these…

Q: A culture initially has P0 number of bacteria. At t = 1 hour the number of bacteria is measured to…

A: A first order initial value problem is a system of equations of the type  F(t,y,y’)=0 ,  y(t0)=y0 .…

Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: Why is a block of agar inoculated rather than streaking the fungal culture on a plate?

A: The culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth…

Q: Why do you have to boil the agar solution before dispensing it into tubes?

A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…

Q: Why bleach disinfectant will the most effective against probiotic?

A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…

Q: What makes a culture media DIFFERENTIAL? Explain and provide examples of differential type of…

A: Answer: Culture media is the growth medium or culture medium which is solid, liquid, or semisolid in…

Q: ating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli,…

A: Aseptic transfer means transferring living microbes from one place to another without contamination…

Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?

A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…

Q: Why does a streaking method used to inoculate plates result in isolated colonies?

A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…

Q: Gram stain of Nodule Suspension Describe the morphology of the crushed nodules under microscopes…

A: The bacteria are stained with the gram stain and can be divided into two groups on the basis of the…

Q: Which of the following would you most expect to find in a fecal contaminated water sample?…

A: Water pollution is described as the fouling of bodies of water. Water pollution occurs when rivers,…

Q: Define a pure culture.

A: A microbiological culture, or microbial culture, is a strategy for duplicating microbial life forms…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…

A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…

Q: Why after a certain period the growth rate of the culture decreases until growth stops?

A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…

Q: A broth culture is initially inoculated with a single bacterial cell. For the first hour the…

A: * Given that  The bacteria has generation time of 30 minutes. So it takes about thirty minutes to…

Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…

A: Microbiology is the branch of science which deals with the study of different types microorganisms…

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?

A: Culture media, also called as growth media, are particular combinations of nutrients and other…

Q: What makes a culture media as SELECTIVE? Explain and provide examples of selective type of culture…

A: A culture medium or growth medium is a substance that is used to grow the microorganisms under…

Q: Why is it important to follow sterile technique?

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Why is it important to use a sterilized loop between streaks when preparing a streak­plate?…

A: Streak plates are prepared to grow and isolate individual colonies of bacteria. Here, we will mark…

Q: What is the correct shape of the unknown bacterium growing on your mannitol salt agar

A: Mannitol salt agar also known as MSA is commonly used selective agar in microbiology. This material…

Q: why is it important that a small amount of inoculum be used in preparation of an agar slant

A: Agar is a jellylike substance derived from the purified cell walls of red algae. It is added to…

Q: Would you use an inoculating loop to transfer bacteria to an agar deep tube?

A: Agar deep tube is a glass tube which is filled with solid medium. The medium is solid due to…

Q: What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for…

A: Enrichment culture media is the one which is used for the growth and isolation of a specific type of…

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…

A: Preparation of culture media and inoculation are two of the very sensitive steps in any…

Q: What is a pure culture and how can one be obtained

A: Cells are the structural and functional units of life.

Q: If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were…

A: Dilution Factor is the factor by which the original solution is diluted. It may be expressed as the…

Q: In your daily life, you utilize aseptic technique in order to minimize contamination. List five…

A: The aseptic technique in a microbiology lab is a must to get the tests right by avoiding…

Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium

A: Growth and morphogenesis of plant is determined by the composition of culture media. There are few…

Q: Bacterial cells can form colonies on the following media EXCEPT: Select one: Nutrient broth Agar…

A: The nutrient broth is the liquid nutrient media that lacks the solidifying agent. It is used for…

Q: Why does dry heat require higher temperatures for longer time periods to sterilize than moist heat

A: Sterilization may be defined as the process which help in the complete removal of all…

Q: The culture primarily contains nutrients such as sugars and a gel (agar). Why don’t viruses grow in…

A: Culture media are an integral part of the microbiology lab. There are various kinds of media used in…

Q: The main of objective of using 13-Line streaking method is to get:

A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…

Q: ow can one be sure that the culture media are sterile or free from contamination

A: Sterility, often known as sterility, is the state of being free of all contaminants and…

Q: Could humans exist on chemically defined media for 48 hours?

A: A chemically defined medium is a growth medium whose components and their concentration (or…

Q: Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the…

A: Microorganisms are present in abundance in nature. When there are no aseptic conditions then…

Q: Why is low microbial population in milk samples a limitation for microscopic count?

A: The bacteria present in milk are lactococcus , lactobacillus, streptococcus, leuconostoc and…

Q: How many different types of colonies can you find on the blood agar plate? describe each. (As shown…

A: Blood agar is general medium that is provided with the nutrients and can be use as a general growth…

Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix.…

A: In serial dilutions, dilutions are multiplied at each step. We can calculate dilution by dividing…

Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…

A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…

Q: What are the primary uses of the following preparations of culture media? Agar regular slant Agar…

A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…

Q: What is a starter culture and what is its purpose? What are the characteristics of a good starter…

A: STARTER CULTURE:- Starter culture are those microorganisms that are used in the production of…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.