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Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet only?.
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- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?
- How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium?What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
- In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?In preparing bacterial smear, why do we need to pass the slide over the flame? Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? Conclusion?Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 X
- Isolating a pure culture from a mix sample is typically done on solid agar but can be performed using a liquid media. How can you isolate a pure culture from a mixed culture in a liquid medium (broth, not agar)?What is the difference between Giemsa and Wright stain?Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?