Q: What is the purpose of the alcohol (OH) in the gram staining process?
A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…
Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…
A:
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: Crystal violet is an example of what type of stain?
A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…
Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…
A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…
Q: List other fluorescent stains. What is the usefulness of each?
A: Fluorescent stains/dyes are non-protein molecules that are employed in visualizing samples under a…
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
A: The simple dye is used in the technique of simple staining to highlight only the specific structures…
Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…
A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…
Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: During acid fast staining first of all heat is used to fix the bacterial smear onto the slide.…
Q: Why is it necessary to use a 24-hrold culture in gram staining?
A: Note: Since you have posted multiple independent questions in the same request, we will solve the…
Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…
A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.
A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…
Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…
A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…
Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a…
A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from…
A: Spores are cellular structures produced by plants, bacteria, and fungi. Spores can be produced for a…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…
A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…
Q: With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation? What…
A: Gram stain Gram staining is a method of staining which is used to classify the bacterial species…
Q: Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…
A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…
Q: What is the purpose of iodine in the Gram stain? To act as a counterstain None of the other…
A: There are various staining techniques that are used to stain different cellular structures. The Gram…
Q: The decolorizer used in the Gram Stain, and the decolorizer used in the Acid Fast Stain, are the…
A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…
Q: Match the following regarding the Gram stain.
A: Depending upon the structure of cell wall, bacteria are divided into two major classes. The basis of…
Q: The Gram staining procedure involves multiple steps with use different reagents. Complete the table…
A: Step 1 Christian Gram in 1884 developed a technique of staining bacteria called Gram staining.…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Regarding the Gram stain procedure: whether Gram-positive or Gram-negative, cells may appear…
A: Q. Regarding the Gram Strain procedure: Whether Gram positive or Gram negative,cells may appear…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: The staining is done to enhance the visualization of cell and certain cellular component under a…
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: Evaluate the following preparations in terms of providing informationon microbial size, shape,…
A: Staining is an important step in observing the microbes under a microscope. they allow to the…
Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…
A: Answer First option Non-acid-fast microbes appear blue in a completed acid-fast stain.
Q: What color are Gram negative cells after decolorizing with ethanol? What is the best age for your…
A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…
A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…
Q: The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in…
A: Acid fast bacteria are those which responds to acid fast staining . Most of the members of…
Q: Gram-staining
A: Reagents using in Gram-staining are : Crystal violet Iodine Alcohol/Acetone Safranin
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: What staining technique is used here? What is the primary stain, decolorizing agent, and counter…
A: Gram Staining is the common, important, and most used differential staining techniques in…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
Q: For each of the following stains, list each of the chemicals used (in order), briefly describe what…
A: In Microbiology staining is needed in order to properly visualize the internal membranes of the…
Explain why only gram-negative cells undergo decolorization during the
Cite the purpose of each of the following reagents in a differential staining procedure.
- Primary stain
- Mordant
- Decolorizing Agent
- Counter stain
What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
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- The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?In preparing bacterial smear, why do we need to pass the slide over the flame? Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? Conclusion?In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?
- Name all the reagents used in Gram-staining and their roles in the staining procedure.Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. DecolorizationWhat is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observed
- Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 1. Simple StainingWhich of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.what effect would failing to drain the smear in-between staining have on your judgment of the gram reaction of an organism?
- What color would gram positive cells appear if ghr safranin staining step was left out of staining procedure ? Why?Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 XDescribe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each component work? (ie. What does the mordant do?)
