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Why is the time of exposure and temperature required for sterilization greater for dry heat than for autoclaving?
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- Can culture media be sterilized in the hot air sterilizer? Why or why not?When is sterilization by filtration preferred over sterilization by heat? Why? What makes heat an effective sterilizing agent? Why are the conditions required for sterilization greater for dry heat (160-180°C for 1-2hrs) than for steam (121°C for 15min)?Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that hinder effective sterilization through autoclaving?
- What is the most difficult type of microbial cell or structure to kill? What methods can be used to sterilize a sample contaminated with these?Why should you filter some liquids for sterilization, rather than autoclaving them?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?
- when heat treatment is used as to contril microbial growth, what is the immediate impact of this increased temperature?Why is chlorination, although beneficial in terms of bacterial pathogen control, of environmental concern?Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.