Q: Identify and describe the 4 wet-heat methods that are used to control microbial growth.
A: The different methods used for controlling the growth of microbes by moist heat are boiling,…
Q: What is the pathogen that exhibits the greatest resistance to heat treatment is the bacteria ?
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: How might an enrichment culture be used to isolate bacteria capable of growing photoautotrophically…
A: Answer: Introduction: Enrichment cultures can be able to rise a less quantity of required organisms…
Q: How does pasteurization help us in our everyday life even before it was discovered?
A: As recently as the nineteenth century, humans risked serious illness or even death by drinking…
Q: Differentiate moist heat from dry sterilizing heat.
A: Dry heat sterilization:-one of the oldest techniques of sterilisation is dry heat sterilisation of…
Q: What is the effect of moist heat and dry heat? Define TDT and TDP. What is the minimum sterilization…
A: Sterilization is defined as a technique which is used for complete removal, killing or deactivation…
Q: when heat treatment is used as to contril microbial growth, what is the immediate impact of this…
A: The process of killing microbes or preventing their growth is called as sterilization. Sterilization…
Q: Identify applications for ethylene oxide sterilization.
A: KEY WORDS :- Ethylene oxide - It is a gas which is flammable in nature and colorless which is used…
Q: Why is heat an effective sterilizing agent?
A: Heat Energy : It is a form of energy which takes place by the movement of the minute particles…
Q: How can a life cycle of microorganism and heat resistance graph be used to determine the boiling…
A: Heat is one of the effective as well oldest physical methods that have been employed for microbial…
Q: Why is the time of exposure and temperature required for sterilization greater for dry heat than for…
A: Any technique that removes, destroys, or deactivates all forms of life and other biological agents…
Q: What temperature is considered adequate for achieving sterilization of a compost pile?
A: temperature considered for achieving sterilization of a compost pile is 135-160 F. At a…
Q: How is microbial growth directly dependent on temperature?
A: Microbes are minute, unicellular/multicellular, prokaryotic living organisms that cannot be seen…
Q: Why does a population of microbes not die instantaneously when exposed to an antimicrobial agent?
A: Antimicrobial agents are agents that are used to stop or prevent the growth of microbes. They…
Q: What is the correlation between radiation “energy” and the mechanisms of sterilization?
A: Safety is of utmost importance while working with any contagious or pathogenic substance or living…
Q: How alcohol is effective at contolling microbial growth?
A: Sterilization: Sterilization includes the complete wipeout of all microscopic life on a surface.…
Q: Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that…
A: Autoclave is an instrument or device which is used in method of moist heat sterilisation; while…
Q: What is the principle behind sterilizing media before use and before disposal
A: Microorganisms may be beneficial or harmful. The harmful microorganism causes many infections which…
Q: What are some advantages and disadvantages of using ionizing radiation to sterilize food?
A: Sterilization involves physical methods for removing or controlling the microbes. The physical…
Q: What settings and time are normally used for sterilization in an autoclave?
A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…
Q: What is an alternative to autoclaving for sterilizing heat-sensitive materials?
A: Any process that kills, eliminates, deactivates, or removes all forms of microorganisms and…
Q: Why do you think autoclave sterilization of media is preferred over open flame?
A: An autoclave is used to sterilize surgical equipment, laboratory instruments, media and other…
Q: Explain why it is essential that bacteria cultures be sterilized in an autoclave.
A: The microbial cultures are grown in the laboratory so as to multiply them or increase the number of…
Q: Are you able to identify the lag, log, stationary and death phase in using this growth curve?
A: Microbes grown in a closed or batch culture in which certain conditions are that no nutrients are…
Q: Why is Escherichia coli used as an indicator organism inmicrobial analyses of water?
A: The well-known gram-negative bacteria are E. coli (Escherichia coli).
Q: Propose a confirmatory procedure that will determine if the microbial growth recovered from canned…
A: A microbe is a living entity that is so tiny that it cannot be seen with the naked eye. Microbiology…
Q: Why must water be subjected to microbiological analysis?
A: Water is used for various purposes in daily lifestyle including food. Thus, it should be monitored…
Q: What are the possible ways to sterilize heat labile products? Explain your answer with suitable…
A: Heat labile materials are those substances that can undergo change rapid change when exposed to high…
Q: Distinguish heat sterilization and "commercial sterility".
A: lowering the prevalence of harmful bacteria until they are no longer a health threat. usually…
Q: What is the point of the pressure in the autoclave sterilization?
A: Autoclaves are the devices which are like pressure cookers and used in laboratories that provide a…
Q: Discuss the principle of flame sterilization.
A: Sterilization is any method that eliminates, destroys, or kills any life such as fungi, bacteria,…
Q: What physical and chemical conditions are necessaryfor the rapid microbial degradation of oil in…
A: Biodegradation refers to the transformation of complex organic compounds by bacteria, fungi, and…
Q: What is an advantage of in-situ bioremediation as a means of eliminating contaminants over chemical…
A: Bioremediation technology is a waste management process which employs various live organisms for…
Q: selective agar media for gram-positive bacteria?
A: Phenyl Ethyl Alcohol Blood Agar is a selective media for gram positive bacteria.
Q: Why is egg enrichment not added to BPA prior to sterilization?
A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…
Q: What products are sterilized using high energy ionizing radiation?
A: Sterilization is the process of killing microorganisms.
Q: What is the difference between assimilatory nitrate reduction and denitrification? Which reaction is…
A: Nitrogen gas is present in the inert form in the atmosphere.
Q: What type of media components cannot be sterilized in an autoclave?
A: Sterilization refers to the complete destruction or elimination of all viable organisms. The process…
Q: What are the different Microbiological sterilization techniques used for Microbiology equipment and…
A: Microbiology is the branch of biology that deals with the study of microorganisms such as bacteria,…
Q: How to reduce or eliminate aerial contamination inside the laboratory?
A: Indoor air quality (IAQ) : To main the indoor air quality becomes an important aspect especially in…
Q: Write the limitations of sterilization by Dry heat?
A: The dry heat sterilization technique includes the removal or killing the microorganism and require…
Q: Why is chlorination, although beneficial in terms of bacterial pathogen control, of environmental…
A: Chlorination is a disinfection procedure in which microorganisms are killed by the use of chlorine…
Q: If you suspect that your microbial culture growing in nutrient broth is contaminated, what can you…
A: The contamination refers to the growth of one or more undesired cultures in your pure sample. It can…
Q: Briefly discuss any two characteristics of microorganisms that should be considered when assessing…
A: The study of microorganisms like bacteria, viruses and fungi is called microbiology. Microbiology…
Q: Are you able to identify the lag, log, stationary and death phase in using this growth curve? Are…
A: The growth curve of bacterial cells can be divided into four distinct phases known as lag, log (or…
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- Drying is an effective antimicrobial strategy because:(a) It oxidizes cellular macromolecules(b) It limits the amount of water available to microbes(c) It increases susceptibility to UV irradiation(d) It triggers the thermal death point1(a)What is a psychrotroph? (b)From what natural sources would you isolate a thermophile? A psychrophile? (C)How does temperature affect the growth of a microorganism? (D)State the temperature class for Escherichia coli, Bacillus sp, Aeromonas sp, Micrococcus luteus, and suggest their optimum growth temperature. 2 (a)Why is dilution important when determining microbe number? (B)How does a decrease in dye colour intensity affect the microbe ? (C)State the possible sources of error if plate counts and colour intensity of dilutions are incorrect or Precautions taken to prevent this from happening. ( this is not a graded assignment)1.) During a laboratory activity, Chemical reactions from mixture of chemicals should be observed under which laboratory equipment? a.) autoclave b.) fume hood c.) Labaratory Safety cabinet d.) Centrifuge 2.) Which type of equipment contains a HEPA filter that only removes suspended materials from exhausted air? a.) Fume hood b.) Biosafety Cabinet I c.) Biosafety Cabinet II d.) Biosafety Cabinet III
- Name 2 indirect methods for measuring microbial growth and describe how each is performed. a. i. Advantage: ii. Disadvantage: b. i. Advantage: ii. Disadvantage:9. The process in which all organisms and pathogens are destroyed is called ------. a) Disinfection b) Sterilization c) Antisepsis d) Pasteurization8. A retort pouch is: a) Filled first with food product and then retorted (heat-sterilization) to extend product shelf life. b) Food is heat-sterilized first, and then added to a pouch under nitrogen c) Retort pouches requires thermally stable seals d) Both a and c
- 1. Define the following terms: a. Chermolithoheterotroph b. Microaerophile c. Chermoorganoheterotroph d. Obligate anaerobe e. Facultative anaerobic f. Coliform1. The selective ingredient in mannitol salt agar is ______________, which selects for the growth of Staphylococcus organisms.1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C. Autoclave d. Sun light
- which of the following is not a chemical method of microbial control? a. soaps and detergents b. hydrogen peroxide c. filtration d. food preservationsOf what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?1. a. Disk A b. Disk B c. Disk C d. Disk D 2. a. Strip A with MIC at 0.25 μg/mL b. Strip B with MIC at 0.064 μg/mL c. Strip C with MIC at 0.075 μg/mL d. All of the strips show equal antimicrobial efficacy