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- Describe the effects of competitive, uncompetitive, and mixed inhibitors onKM and Vmax.The effect of uncompetitive inhibitor is reversed by increasing substrate concentration?Rank these substrates in order of best inhibitor (highest binding affinity) to worst inhibitor (lowest binding affinity). Rank from highest binding affinity to lowest binding affinity. To rank items as equivalent, overlap them.
- Select all that are correct. The minimum inhibitory concentration a. can be determined by a Kirby-Bauer disk diffusion testb. can be determined by a dilution series in nutrient broth c. is the highest amount of inhibitory substance that can be safely administered d. is the lowest amount of inhibitory substane that prevents bacterial multiplication e. is the lowest amount of a chemical that kills all the organismsDefine the following terms: a. competitive inhibitor b. uncompetitive inhibitor c. noncompetitive inhibitor d. reversible inhibitor e. irreversible inhibitorDraw and label the Lineweaver–Burk, Plots for each type of inhibitor.
- Why are uncompetitive and mixed inhibitors generally considered to be more eff ective in vivo than competitive inhibitors?Inhibitor X exerts which of the following effects on the above enzyme (lactase)? (inhibitor X changes lactase activity to a Vo of 0.10 mM per minute when [S] = 1.0 mM, and a Vo of 0.133333333333 mM per minute when [S] = 2.0 mM) pure non-competitive inhibition uncompetitive inhibition competitive inhibition all of the above none of the aboveWhat effect is seen on a Lineweaver-Burke graph when a non-competitive inhibitor is added to an enzymatic reaction? the y-intercepts changes the x-intercepts changes and the slope of the line changes the y-intercepts changes and the slope of the line changes the x-intercepts changes the slope of the line changes
- State and describe the three different types of inhibitors. short answer please.Years ago, uncouplers were suggested to make wonderful diet drugs. Explain why this idea was proposed and why it was rejected. Why might the producers of antiperspirants be supportive of the idea?A drug with which of the following molecular mechanisms of action would be least likely to function as a DHFR inhibitor? Group of answer choices A) Block substrate from binding to the DHFR active site B) Alter folding to increase NADPH cofactor binding C) Increase DHFR degradation