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You are studying a new fungus, G. lucasus, and are interested the molecules that control the cell cycle of this new organism. You make some mutant cells and find an intriguing mutant that has two mutations - one in the force gene and one in the darkside gene. a. You perform propidium-iodide cell cycle analysis by flow cytometry on wild-type and the double mutant cells and your results are below. During what stage(s) of the cell cycle are the double mutant cells arresting their growth? Please briefly explain your answer. Wild-Type Cells # of cells 180 kDa 110 kDa 80 kDa b. In studying the gene products of the force and darkside genes, you determine that the force gene produces a polypeptide of 80 kDa, and the darkside gene produces a polypeptide of 42 kDa. You collect lysates from wild-type cells in G₁, S, or G₂/M phases of the cell cycle and perform a Western blot (immunoblot) with either an antibody against force or an antibody against darkside... only you forget to label your blots (rookie mistake!). 60 kDa 42 kDa 2 relative DNA content 30 kDa # of cells Double-Mutant Cells Gel #1 Wild-Type Cells in: Ladder G₁ SG₂/M 2 relative DNA content 180 kDa 110 kDa 80 kDa 60 kDa 42 kDa 30 kDa Gel #2 Wild-Type Cells in: Ladder G₁ S G₂/M i) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin-dependent kinase (Cdk)? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion. ii) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion.

You are studying a new fungus, G. lucasus, and are interested the molecules that control the cell cycle of this new organism. You make some mutant cells and find an intriguing mutant that has two mutations - one in the force gene and one in the darkside gene. a. You perform propidium-iodide cell cycle analysis by flow cytometry on wild-type and the double mutant cells and your results are below. During what stage(s) of the cell cycle are the double mutant cells arresting their growth? Please briefly explain your answer. Wild-Type Cells # of cells 180 kDa 110 kDa 80 kDa b. In studying the gene products of the force and darkside genes, you determine that the force gene produces a polypeptide of 80 kDa, and the darkside gene produces a polypeptide of 42 kDa. You collect lysates from wild-type cells in G₁, S, or G₂/M phases of the cell cycle and perform a Western blot (immunoblot) with either an antibody against force or an antibody against darkside... only you forget to label your blots (rookie mistake!). 60 kDa 42 kDa 2 relative DNA content 30 kDa # of cells Double-Mutant Cells Gel #1 Wild-Type Cells in: Ladder G₁ SG₂/M 2 relative DNA content 180 kDa 110 kDa 80 kDa 60 kDa 42 kDa 30 kDa Gel #2 Wild-Type Cells in: Ladder G₁ S G₂/M i) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin-dependent kinase (Cdk)? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion. ii) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion.

Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter10: Cell Division And Mitosis
Section: Chapter Questions
Problem 1ITD: Biologists have long been interested in the effects of radiation on cells. In one experiment,...
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You are studying a new fungus, G. lucasus, and are interested the molecules that control the cell cycle of this new organism. You make some mutant cells and find an intriguing mutant that has two mutations - one in the force gene and one in the darkside gene. a. You perform propidium-iodide cell cycle analysis by flow cytometry on wild-type and the double mutant cells and your results are below. During what stage(s) of the cell cycle are the double mutant cells arresting their growth? Please briefly explain your answer. # of cells Wild-Type Cells In 180 kDa 110 kDa 80 kDa b. In studying the gene products of the force and darkside genes, you determine that the force gene produces a polypeptide of 80 kDa, and the darkside gene produces a polypeptide of 42 kDa. You collect lysates from wild-type cells in G₁, S, or G₂/M phases of the cell cycle and perform a Western blot (immunoblot) with either an antibody against force or an antibody against darkside ... only you forget to label your blots (rookie mistake!). 60 kDa 42 kDa relative DNA content 30 kDa # of cells Gel #1 Wild-Type Cells in: S G₂/M Ladder G₁ Double-Mutant Cells relative DNA content 180 kDa 110 kDa 80 kDa 60 kDa 42 kDa 30 kDa Ladder Gel #2 Wild-Type Cells in: G₁ S G₂/M i) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin-dependent kinase (Cdk)? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion. ii) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion.
Transcribed Image Text:You are studying a new fungus, G. lucasus, and are interested the molecules that control the cell cycle of this new organism. You make some mutant cells and find an intriguing mutant that has two mutations - one in the force gene and one in the darkside gene. a. You perform propidium-iodide cell cycle analysis by flow cytometry on wild-type and the double mutant cells and your results are below. During what stage(s) of the cell cycle are the double mutant cells arresting their growth? Please briefly explain your answer. # of cells Wild-Type Cells In 180 kDa 110 kDa 80 kDa b. In studying the gene products of the force and darkside genes, you determine that the force gene produces a polypeptide of 80 kDa, and the darkside gene produces a polypeptide of 42 kDa. You collect lysates from wild-type cells in G₁, S, or G₂/M phases of the cell cycle and perform a Western blot (immunoblot) with either an antibody against force or an antibody against darkside ... only you forget to label your blots (rookie mistake!). 60 kDa 42 kDa relative DNA content 30 kDa # of cells Gel #1 Wild-Type Cells in: S G₂/M Ladder G₁ Double-Mutant Cells relative DNA content 180 kDa 110 kDa 80 kDa 60 kDa 42 kDa 30 kDa Ladder Gel #2 Wild-Type Cells in: G₁ S G₂/M i) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin-dependent kinase (Cdk)? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion. ii) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion.
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