You arrived in Butte County after the Camp Fire devastated the community of Paradise and the surrounding area. In the shelters, there is an outbreak of Norovirus causing diarrhea. You take food and tap water/drinking water samples from several sources. Which medium would you use to select for the growth of Gram-negative fecal bacteria from the food and water samples? TSA O MacConkey O Sab Blood Agar
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
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