You come across a gram positive bacterium with the following nucleotides: ATTCGATCGGTATAT. This bacterium most likely is a [ Select one] Firmicute Actinobacterium Proteobacterium You know this because of the [ Select one ] A+T G+C A+C G+T
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- A swollen can of chicken soup is examined by the public healthlaboratory and found to contain large numbers of gram-negative,H2S-positive bacilli. Which biochemical tests would you perform to identifythe genus of the contaminant? Justify your test choices.Name ONE glycopeptide and state what this glycopeptide binds to within a named bacterium and how this kills the bacteriumA certain medium has the following composition:Glucose 15 gYeast extract 5 gPeptone 5 gKH2PO4 2 gDistilled water 1,000 mla. Tell what chemical category this medium belongs to, and explainwhy this is true.b. How could you convert Staphylococcus medium intoa nonsynthetic medium?
- MacConkey agar is often used to differentiate E. coli from other Gram-negative rods. The recipe for MacConkey agar is given in the table. Suppose you plated a mixed culture of Escherichia coli, Bacillus subtilis, and Streptococcus pyogenes onto a MacConkey agar plate. Which species would grow, and how would they appear? Why would the others NOT grow?Which of the following affects the elimination of bacteriafrom an object?(a) Number of bacteria present(b) Temperature(c) pH(d) Presence of organic matter(e) All of the aboveSuppose you would like to determine the number of bacteria in a culture via anaerobic plate count. Explain (with pictures)how you would set up a dilutionseries (10-4,10-6, 10-8) usingthe fewest number of dilution blanks.Draw andlabel your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated.
- A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table above) into a non-synthetic medium? a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? One that lives in the human stomach? Why are intestinal bacteria able to grow on media containing bile?. A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…Ammonia is oxidised to nitrate with the help of which bacteria?1.Nitrobacter2.Nitococcus3.Nitrogenase
- Calculate the surface/ volume ratio of the spherical Neisseria gonorhoe bacterium. Compare this with the surface -to-volume ratio of the large eukaryote ameba Neisseria gonorhoe bacterium = 0.5 micrometer in diameter eukaryote ameba = 150 mm in diameterWhat will be the results of the following experiment with explanations: A. Gram-positive bacteria and Gram-negative bacteria were placed into 0.2% NaCl solution. B. Gram-negative bacterial isolate was placed into 0. 3% NaCl solution containing only Lysozyme enzyme. C. Gram-positive bacteria and Gram-negative bacteria were placed into 0.09% NaCl solution containing only Penicillin?If an acid-fast bacterium is detected in a clinical sample, thenthe organism could bea) Cryptococcus neoformans .b) Mycobacterium tuberculosis .c) Neisseria gonorrhoeae .d) Streptococcus pneumoniae .e) Streptococcus pyogenes .