A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table above) into a non-synthetic medium? a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean?

A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table above) into a non-synthetic medium? a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean?

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

See similar textbooks

Similar questions

. A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…
If the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be accommodated, in principle, in 5 mL of saturated culture? (1 mL = 1 cm3). Show your calculations.
A certain medium has the following composition:Glucose 15 gYeast extract 5 gPeptone 5 gKH2PO4 2 gDistilled water 1,000 mla. Tell what chemical category this medium belongs to, and explainwhy this is true.b. How could you convert Staphylococcus medium intoa nonsynthetic medium?
A bacterium has a generation time of 30 minutes. You transfer cells from an exponentially growing culture to a fresh source of the same medium so that the freshly inoculated medium contains 3.2 x 106 cells per milliliter. This new culture does not exhibit a lag phase. About how many cells (per milliliter) should you have after 1.5 hours incubation?? Solve this step by step and by using formula
Suppose you have a bacterium, which is normally killed in an hour when growing in a medium containing penicillin. This bacterium is an auxotroph and requires tryptophan for growth. When placed in a medium lacking both tryptophan and penicillin, the bacterium neither grows nor dies for several hours. Question: Is it killed in an hour in a medium lacking tryptophan but containing penicillin? (Yes/No and explain briefly why).
Why are the Methyl Red/Voges-Proskauer tests done after 48 hours of incubation?a) Readings taken at earlier time points may be different from readings taken at 48 hours b) Bacteria need 48 hours to process all the oxygen in the media c) Bacteria grow slowly in the MR/VP media d) K2HPO4 needs 48 hours to react with the acids produced by fermenting bacteria
The ingredients in the MR/VP Broth are: Peptone (protein) 7g Glucose 5g Phosphate buffer 5g Distilled water 1L Examine the above figure. This figure shows how the pH in the Methyl Red and Voges-Proskauer (MR-VP) broth changes during incubation from time zero to 48 hours with a VP-positive bacteria species. VP-positive bacteria initially produce lactic acid and temporarily lower the pH, but the lowered pH activates the 2,3-butanediol fermentation pathway. Accumulation of its neutral end products such as acetoin and 2,3-butanediol raises the pH by the end of the incubation time. The acetoin that accumulates in the broth also reacts with the reagents added after incubation to produce a red color. If the reagents were added and the results were recorded early, at 24 hours rather than at the full incubation time at 48 hours, would the color result for the VP (+) bacteria be the same at 24 hours than at 48 hours? If different, explain how the color result is different. What chemical…
A. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media? B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?
Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…
Helicobacter pylori, a bacterium that causes stomach ulcers, can grow in medium with a pH above 5. It can survive but not grow in pH 2 medium as long as the medium contains Urea. You inoculate a pH 2 broth with 1,000,000 H. pylori and for 8 hours the organism did not grow. The next morning however, the medium has become quite turbid, suggesting that the organism has grown. Explain how this could happen. What phylum and class does this organism belong?
You measure your E. coli and find them to have an OD600 of 1.20. You have 50 ml of culture, and you know that there are 2.5x109 cells per 1.0 OD600. Based on this information, how many E. coli cells are present in the culture you’re about to centrifuge?
Your animal cell culture laboratory suddenly suffered from a bacterial contamination. Several components like vitamins, yeast hydrolysate, and fetal calf serum are temperature sensitive and must be filtered through a bacteriological filter to add to the autoclaved animal cell culture media. After careful considerations, you found that there are no issues with the autoclaving, but there is something wrong with the filter sterilization process, and changing new batches of filter did not remove the bacterial contamination. Which of the following rationale best describes the probable cause? Group of answer choices A. The media is probably contaminated with E. coli, which passed through the 0.2-micron bacteriological filter. B. The media is probably contaminated with Mycoplasma, due to the lack of cell walls they are the only bacteria that can pass through the 0.2-micron bacteriological filter. C. All of the other answer choices could be correct D. The media is probably…