You infect bacteria with two phages during a cis test. This produces a lysate that contains phage particles. Before you can count outcomes, you need to work out the amount of phage to use in subsequent experiments. You start by making two ten- fold serial dilutions of the lysate and then add 1mL of the final diluted lysate to a plate of permissive strain e.coli. You observe 10 plaques. How many phage per mL were present in the original lysate? O a. 10 phage/mL o b. 3,300 phage/mL O c. 10,000 phage/mL o d. 1,000 phage/mL e. 33 phage/mL

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter17: Bacterial And Viral Genetics
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You infect bacteria with two phages during a cis test. This produces a lysate that contains phage particles. Before you can
count outcomes, you need to work out the amount of phage to use in subsequent experiments. You start by making two ten-
fold serial dilutions of the lysate and then add 1mL of the final diluted lysate to a plate of permissive strain e.coli. You observe
10 plaques. How many phage per mL were present in the original lysate?
a. 10 phage/mL
o b. 3,300 phage/mL
c. 10,000 phage/mL
o d. 1,000 phage/mL
e. 33 phage/mL
Transcribed Image Text:You infect bacteria with two phages during a cis test. This produces a lysate that contains phage particles. Before you can count outcomes, you need to work out the amount of phage to use in subsequent experiments. You start by making two ten- fold serial dilutions of the lysate and then add 1mL of the final diluted lysate to a plate of permissive strain e.coli. You observe 10 plaques. How many phage per mL were present in the original lysate? a. 10 phage/mL o b. 3,300 phage/mL c. 10,000 phage/mL o d. 1,000 phage/mL e. 33 phage/mL
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