There are two figures- one for vector control plate & another PCRinsert+vector experimental plate. Control plate = 10 blue colonies , Ligation plate ( experimental) = 150 white , 8 blue. Interpret what each figure means, what is its importance (control and experimental)? Why do colonies grow on vector control plates? Why are both blue and white colonies in your experimental plate? What were the components of the agar plate & their use (AMP+Xgal+IPTG)? What do these suggest about amp sensitivity and resistance? Why did we use X-gal and IPTG? Can you think of any issue with your result? Is the recombination efficiency good? Is your transformation efficiency good? •Write a concluding sentence about this lab and the data you analyzed.
•There are two figures- one for vector control plate & another PCRinsert+vector experimental plate. Control plate = 10 blue colonies , Ligation plate ( experimental) = 150 white , 8 blue.
Interpret what each figure means, what is its importance (control and experimental)? Why do colonies grow on vector control plates? Why are both blue and white colonies in your experimental plate? What were the components of the agar plate & their use (AMP+Xgal+IPTG)? What do these suggest about amp sensitivity and resistance? Why did we use X-gal and IPTG? Can you think of any issue with your result? Is the recombination efficiency good? Is your transformation efficiency good?
•Write a concluding sentence about this lab and the data you analyzed.
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