You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.
Q: What do urea, enzymes and ammonia all have in common? they are all proteins O phosphorous they are…
A: Urea is used as a fertilizer it is formed in our bodies as a waste product.Ammonia is also a waste…
Q: please answer question by filling in blanks of image in question
A: In this exercise, we're exploring the results of a genome-wide association analysis of gout, as…
Q: Can you help me create a dichotomous key for these miccoorganisms and tests? Micoorganisms:…
A: A dichotomous key is a tool used in taxonomy to identify and classify organisms based on specific…
Q: Although DNA polymerases require both a template and a primer, the following single-stranded…
A: DNA polymerases require both a template and a primer to synthesize DNA. However, some…
Q: Chemical synapses take longer to transmit a signal than electrical synapses. True False
A: Chemical synapses operate by releasing neurotransmitter molecules from the presynaptic neuron's axon…
Q: When a neuron is in the relative refractory period, a stimulus has to be stronger than normal to…
A: In the phase of the neural action potential cycle known as the relative refractory period, a neuron…
Q: Design an experiment that will allow to determine if there is fungal adaption to thermal soils Draw…
A: Fungi are a diverse group of microorganisms that can be found in all types of environments,…
Q: Fill in the blanks below using the following words: Sensory, motor, mixed, muscle, and spinal cord.…
A: Neurons are tiny, specialized cells in the brain and nervous system that help us think, feel, and…
Q: Along with mutation, which of the following is another way that bacteria acquire antibiotic…
A: Mutation is any abrupt change in the DNA sequence. This change may cause the bacteria resistant to a…
Q: A DNA polymerase has everything it needs for extension, EXCEPT for RNA primers. What is the most…
A: DNA Polymerase are group of enzymes that forms new copies of DNA, in the form of nucleic acid…
Q: What are the factos that affect callus formation, especially if it does not require sunlight (root…
A: Callus formation refers to the process in which undifferentiated, often unorganized plant cells…
Q: Could you tell me the factors or things that stimulate gastric emptying? In a previous assignment I…
A: Digestive system is responsible for ingestion, digestion, absorption and assimilation of nutrients…
Q: Imagine you are studying the genetic basis of gout using GWAS and have available molecular markers…
A: In this exercise, we're exploring the results of a genome-wide association analysis of gout, as…
Q: Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg? Write…
A: Escherichia coli is a Gram-negative bacteria. It is present as normal intestinal flora but can also…
Q: Lumbosacral Coccyge nerve Posterior view What does "A" represent? spinal nerves conus medullaris…
A: The spinal cord is a lengthy band of tissue that resembles a tube. It ties your brain and lower back…
Q: In E. coli, the genes for histidine (his), arginine (arg), tyrosine (tyr), and valine (val)…
A: In this scenario, we are looking at the cotransduction of the histidine (his) and valine (val) genes…
Q: Why is p53 considered a tumor suppressor protein? Question 12 options: a) Because p53…
A: p53 is a tumor suppressor protein because it helps to prevent cells with damaged DNA from dividing.…
Q: Which dietary approach is recommended for managing Type 2 diabetes? a) Monitoring the glycemic…
A: Type 2 diabetes is a high blood glucose level condition in the body, where the cells are unable to…
Q: Plot below shows the velocity of the reaction catalyzed by phosphofructokinase-1 as a function of…
A: Glycolysis is the first step of cellular respiration that is responsible for the production of…
Q: A. Describe three major steps in the process of treating a named disease which affects either…
A: The natural structure or function of an organism, such as a person, animal, or plant, can be…
Q: Which of the following is an example of a mutualism? Phoresy (seed transport) Mycorrhizal fungi and…
A: Mutualism is type of relationship in which two organisms of different species work together and also…
Q: Four different Hfr strains of bacteria were used in timed mating experiments. On the circle below,…
A: Hfr cells – these cells are also called high-frequency recombination cells. These types of cell have…
Q: A friend was recently diagnosed with strep throat. One week after their treatment, his symptoms…
A: Strep throat is a bacterial infection caused by a bacterium species known as Streptococcus pyogenes.…
Q: How could we promote rapid or slow signaling for medical purposes? So there are many examples of…
A: Most of the processes that occur inside the cells are due to a kind of response created by a…
Q: UESTION 1 1.1. Describe five extreme environments in which archaea can be found. 1.2. Discuss the…
A: Archaea are remarkable microorganisms known for their ability to thrive in extreme environments that…
Q: Cisplatin is an anticancer drug that exerts its effects by creating DNA adducts and damaging DNA.…
A: Cisplatin is a well-known anticancer drug widely used in the treatment of various malignancies,…
Q: Which bacterial cell structure is a good drug target because of the low risk of damaging ost cells?…
A: Drug target is special type of molecule in the body that is mainly protein, it is associated with a…
Q: A Moving to another question will save this response. Question 8 Neurons look and work different…
A: Neurons and muscle cells are two of the most important types of cells in the human body. Neurons are…
Q: A yellow mouse blends into a yellow sandy beach area while a white mouse stands out. The yellow…
A: According to the idea of evolution in biology, the many species of plants, animals, and other…
Q: A pharmaceutical company just developed a drug (Cytarabine) that inhibits the activity of cytosine…
A: Cytosine deaminase is an essential enzyme for the metabolism of nucleic acids, including the…
Q: It is possible to measure the number of cells in a broth by measuring the amount of light that goes…
A: CFU stands for colony-forming units. This unit is used to estimate the number of live or viable…
Q: Two servings of this product provides g of protein.
A: Q1.Step 1: Identify the protein content per serving.The food label provides the protein content per…
Q: You are recording from a funny-looking cell. You hold the cell at various voltages and record the…
A: This experiment investigates the permeability of a cell to K+ ions. By comparing the current…
Q: 2. Cellular Respiration a. Indicate how many molecules of each type are produced during GLYCOLYSIS…
A: It is the initial phase of both aerobic and anaerobic respiration, and almost all living things…
Q: Folate deficiency in a cancer patient being treated with 5-Fluorouracil will lead to diminished…
A: Imagine 5-Fluorouracil (5-FU) as a superhero trying to stop a villain called "thymidylate synthase"…
Q: Organize the four craniums based on prognathism (reduced to increased prognathism). A. Homo…
A: When compared to the upper face and cranial features, prognathism is the amount to which the lower…
Q: QUESTION 8 Emergent properties of living systems are defined as properties that O are apparent only…
A: A unicellular organism is an organism that consists of only a single cell, such as an amoeba,…
Q: 1The gonadotropins are hormones produced by the anterior pituitary gland and affect both male and…
A: Gonadotropins are hormones generated by the gonadotrophic cells. These hormones are released by the…
Q: DNA was extracted from met leu his pro bacteria and used to transform met leu" his pro bacteria,…
A: The transformation shown produces:pro+ leu+ =colonies pro+ his+ = colonies leu+ his+ =…
Q: THEME 2: Observe the figure below depicting cells undergoing meiosis and indicating which ones have…
A: Meiosis is a sort of cell division that takes place in sexually reproducing organisms and produces…
Q: How do Na + enter a neurone when initiating an action potential? A) voltage- gated Na+ facilitated…
A: A short electrical impulse that passes through the membrane of a muscle or nerve cell is known as an…
Q: The illustration best describes: A dormant bacterial infection A dormant viral infection An…
A: A virus is a tiny infectious agent that can reproduce only inside the living cells of an organism.…
Q: QUESTION 5 Compared to single-unit smooth muscle, multi-unit smooth muscle: none of the other…
A: Smooth muscle is found in the walls of internal organs as it can contract and relax when gets…
Q: Explain any one method of preparation of bio-fertilizers.
A: Fermentation produces biofertilizers, which are effective live soil microbes. They increase plant…
Q: THEME I: Please label and rank the following point mutations in increasing order of their potential…
A: Label and rank of the given mutations in increasing (smaller to larger) order of their potential to…
Q: 1 口 [
A: It is the ventral surface of the sheep's brain which remains enclosed in duramater. In this image…
Q: Using the table, give at least 10 exoskeletons found in animals, source, its description and state…
A: Exoskeleton animals, often referred to as exoskeletal animals or arthropods, are a variety of…
Q: In this situation, the round molecules will diffuse toward the
A: Diffusion is the movement of molecules from an area of high concentration to an area of low…
Q: 1 3 (4) (5) (a) Monocotyledon (Corn)
A: Zea mays, also known as corn, is a monocotyledonous plant, also known as monocot. This indicates…
Q: In a population of pika, you find that individuals vary in their fur color - there are greyish and…
A: According to the Hardy-Weinberg equilibrium concept the allele and genotype frequencies of a…
You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.
Step by step
Solved in 3 steps
- In a gel filtration chromatography, what type of gel must be used when the protein size is 2500 Da? Explain.In a mixture of five proteins listed, draw an elution profile (Absorbance vs. mL eluted, arbitrary) for the purification of the listed proteins on a gel filtration chromatography resin: cytochrome c (pI = 5.4; Mr = 13,000), immunoglobulin G (pI = 7.3; Mr = 145,000), ribonuclease A (pI = 9.6; Mr = 13,700), RNA polymerase (pI = 6.3; Mr = 450,000), human serum albumin (pI = 5.4; Mr = 68,500). Label your elution peaks. Draw a sketch of an SDS-PAGE, reflecting the mobility of the above mixture as they elute from the column. Label you protein bands.A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?
- In nickel column affinity chromatography,how does the protein binds to column?How is the protein eluted from the column?For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. 7. If the red cell suspension is the stock solution, what is being quantified in this test? What diluent/reactant should be used to detect your answer in number 7?4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?
- In the assay of citric acid, what do you think about the nature of solution sample ? a) Before titration and b) After titration.Using Bradford Assay, plot the standard curve and find the unknowns. Protein Concentration A595 of sample 0 0 3 0.220 6.5 0.383 12 0.667 18.8 0.930 24.2 1.090 Unknown 1 1.085 Unknown 2 0.708 Unknown 3 0.193After performing the manual Albumin assay, you get the absorbance value of 0.205 for a 4.5 g/dL albumin standard and 0.114 for control A. What is the calculated value of the control? (just write a number with one decimal, the units will be mg/dL)
- You will be given 1 mL of a 2.0 mg/mL solution of bovine serum albumin (BSA) and 1 mL of a 2.0 mg/mL solution of bovine gamma globulin (BGG). From these solutions, you will need to make 100 μL of each of the following standards using 1x MBP purification buffer as the diluent for preparation of a standard curve: 1000 μg/mL, 750 μg/mL, 500 μg/mL, 250 μg/mL, 125 μg/mL, and 0 μg/mL.What is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm with the presence of the Bradford reagent? (note: explain not less than 5 sentences).What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?