Only do parts d, e, and f You want to do an experiment on cells to treat them with very lowconcentrations of both Atrazine and DMSO (which is the solvent you need to use toDoy make your atrazine stock solution). To do this you must first make a relativelyconcentrated stock of atrazine (3 x106 ppm Atrazine, DMSO) by dissolving powdered vadatrazine in DMSO.2.Next you use the (3 x10° ppm Atrazine, DMSO) stock to make a less concentrated solution bycombining:1 μL of (3 X 106 ppm Atrazine, DMSO)0.999 ml of cell culture mediaVIII u azilit in this C..To get to your experimental conditions you combine the following 2 liquids (consider the finalvolume of this mixture = 60 ml for your calculations).60 μl of the solution you prepared in the highlighted text60 ml of cells and cell culture mediad. What is the concentration of Atrazine in ppm in your experimental conditions?e. What is the concentration of DMSO in %v/v in your experimental conditions?f. Why did you measure 60 ml and not 59.04 ml to make up this final solution?

We must take into account the involved dilutions and volumes in order to determine the atrazine…

You want to do an experiment on cells to treat them with very low concentrations of both Atrazine and DMSO (which is the solvent you need to use to oy make your atrazine stock solution). To do this you must first make a relatively concentrated stock of atrazine (3 x106 ppm Atrazine, DMSO) by dissolving powdered vad atrazine in DMSO. 2. Next you use the (3 x106 ppm Atrazine, DMSO) stock to make a less concentrated solution by combining: 1 μL of (3 X 106 ppm Atrazine, DMSO) 0.999 ml of cell culture media VIIVI Au alilie in uns TIC.. To get to your experimental conditions you combine the following 2 liquids (consider the final volume of this mixture = 60 ml for your calculations). 60 μl of the solution you prepared in the highlighted text 60 ml of cells and cell culture media d. What is the concentration of Atrazine in ppm in your experimental conditions? e. What is the concentration of DMSO in %v/v in your experimental conditions? f. Why did you measure 60 ml and not 59.04 ml to make up this final solution?

You want to do an experiment on cells to treat them with very low concentrations of both Atrazine and DMSO (which is the solvent you need to use to oy make your atrazine stock solution). To do this you must first make a relatively concentrated stock of atrazine (3 x106 ppm Atrazine, DMSO) by dissolving powdered vad atrazine in DMSO. 2. Next you use the (3 x106 ppm Atrazine, DMSO) stock to make a less concentrated solution by combining: 1 μL of (3 X 106 ppm Atrazine, DMSO) 0.999 ml of cell culture media VIIVI Au alilie in uns TIC.. To get to your experimental conditions you combine the following 2 liquids (consider the final volume of this mixture = 60 ml for your calculations). 60 μl of the solution you prepared in the highlighted text 60 ml of cells and cell culture media d. What is the concentration of Atrazine in ppm in your experimental conditions? e. What is the concentration of DMSO in %v/v in your experimental conditions? f. Why did you measure 60 ml and not 59.04 ml to make up this final solution?

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter11: Health Of Aquatic Animals

Section: Chapter Questions

Problem 3KA

See similar textbooks

Similar questions

Anthocyanins are another type of plant pigment that are purple or red. Say you run a chromatography experiment using the same setup as you did in this lab, and you find that anthocyanins do not move from the line of origin. Assuming the solvent traveled the same distance as in your experiment (D in Table 1 above), what is the Rf value of anthocyanin?
You are designing an experiment to test the reaction of potato samples with varying amounts of surface area with equal amounts of hydrogen peroxide. In your experiment, you have two (2) small test tubes, labeled A and B respectively and each filled with 10 ml of hydrogen peroxide. A 1 cm3 cube of potato will be placed into Test Tube A. In Test Tube B, the same volume of potato (1 cm3) will be placed into this test tube, but this cube of potato will be cut into 8 pieces before being dropped into Test Tube B. After 5 minutes, which of the test tubes do you predict will show a greater amount of chemical reaction (the releasing of oxygen gas as seen through fizzing) between the potato sample containing the enzyme catalase and the hydrogen peroxide? Why?
We have 10 mL of cells to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 20 µM. How much of a 30 mM stock solution of H2O2 would we add to our cells? If you could please help me understand the steps of this problem!
Some antacids are used by dropping a tablet into a glass of water, which results in significant fizzing, and then drinking the water. Is there any evidence to suggest there might be a chemical reaction involved? What is the evidence?
Emergency treatment of cardiac arrest victims sometimes involves injection of a calcium chloride solution directly into the heart muscle. How many grams of CaCl2 are administered in an injection of 5.0 mL of a 5.0% 1m/v2 solution? How many milliequivalents of Ca2+?
Specific rotation of L-alanine [(+)alanine) in water (at 25 °C) is +2.8. Suppose you have 2.00 g of mixture of L and D-alanine [(-)alanine]. You dissolved it in 10 mL of water, and measured rotation in 10 cm cell. The observed rotation was +0.12. Calculate the ee% for the mixture. How many grams of D- and L-alanine do you have in the sample?
You want to feed a new flask @: 2 x 105 cells/mL, 8 mL total per flask. Describe how you dilute your cells in CRPMI? Please show the general formula, your specific equation and your calculations.
You are trying to separate a mixture of AMP, ADP, and ATP using ion exchange chromatography. Which of the following is NOT an appropriate reagent for this experiment? You can not separate nucleotides using ion exchange chromatography. A positively charged column Protonated buffer 0.05 M NaCl buffer (constant concentration)
You must make a choice between two chromatographic systems for the analysis of a specific compound. System A shows a partition coefficient of 1.0 and system B a partition coefficient of 0.25. Explain which of the systems will elute your analyte the fastest. Give a full motivation of your answer?
The amino acid alanine has two isomers, α-alanine and β-alanine. When equal masses of these two compounds are dissolved in equal amounts of a solvent, the solution of α-alanine freezes at the lowest temperature. Which form, α-alanine or β-alanine, has the larger equilibrium constant for ionization (HX ⇌ H+ + X−) ?
An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.