You must make a choice between two chromatographic systems for the analysis of a specific compound. System A shows a partition coefficient of 1.0 and system B a partition coefficient of 0.25. Explain which of the systems will elute your analyte the fastest. Give a full motivation of your answer?
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You must make a choice between two chromatographic systems for the analysis of a specific compound. System A shows a partition coefficient of 1.0 and system B a partition coefficient of 0.25. Explain which of the systems will elute your analyte the fastest. Give a full motivation of your answer?
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- Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?SEE EXAMPLE IN THE IMAGE A mixture of 0.20M acetic acid and 0.30M sodium acetate is given. Calculate the pH of the medium if the pKa of the acetic acid is 4.76. Show your complete solution.The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pHA chemist is performing a precipitation titration on a 1.00 L sample containing an unknown concentration of the cadmium ion, Cd2+. If it takes 24.52 mL of a 0.250 M solution of Na2CO3 to reach the end point, calculate the concentration of the cadmium ion?After purification, the chemist wants to know the identity of the unknown compound. The person then prepared three standards and did a TLC experiment. Listed in the table are the distance travelled by the solvent and the compounds. Distance travelled (mm) Sample 32 Standard A 25 Standard B 34 Standard C 17 Solvent 48 A B C Which standard could the unknown compound be? Justify you answer. Show all your calculations.
- A mixture containing only KCl and NaBr is analyzed by the Mohr Method. A 0.3172-g sample is dissolved in 50 mL water and titrated to the Ag2CrO4 endpoint, requiring 36.85 mL of 0.1120 M AgNO3. A blank titration requires 0.71 mL of titrant to reach the same endpoint. Report the % (w/w) KCl and NaBr in the sample. [Ans . 84.41 % (w /w); 17.59 % (w /w)]You have a stock solution of 40% hydrogen peroxide. You need to make several dilutions for a calibration curve. Calculate how much volume of the stock hydrogen peroxide you need to make 50 mL of 11% hydrogen peroxide.What is the purpose of: A. The concentrated salt solution? B. adding isopropyl alcohol to the solution? Why does it need to be in low temperature?
- The standard curve provided below will allow you to answer the remaining questions. Generic Standard Curve 0.7 y = 0.9425x- 0.0034 0.6 0.5 0.4 0.3 02 0.1 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Concentration (mg/ml) - Please describe two ways in which you could use this standard curve to determine the concentration of a pigment in an unknown sample.| #1: #2: -What is your rough estimation of the concentration if you determine the absorbance of a sample is 0.150? AbsorbanceYou dissolve a 3.435 gram sample of potato in acid to determine the levels of solanine (the reason why green potatoes are poisonous) and diluted it to a total volume of 500.0 mL. You have also created a standard curve as outlined below. Solve for the ug/gram concentration of solanine in the potato sample. ppm Solanine Std. Abs 0.213 5 0.315 10 0.423 15 0.532 Unknown sample 0.314 Your Answer:You receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many mL of D50W and sterile water are needed to complete the order? *