Your lab is investigating cellulose degradation by Cellvibrio japonicus and has generated a large number of mutant strains. You use the wild-type (WT) strain as your positive control and an enzyme secretion mutant (gspD) as your negative control. The remaining eight strains have in- frame deletions for genes predicted to encode cellulases. When you grow the strains on filter paper you obtain the following growth data: Filter paper • WT + gspD cel45A cel6A 0.10- + cel5A + cel5B 0.01- 20 80 Filter paper + WT + gpsD + cbp2D e cbp2E + Ipmo10A * Ipmo10B 0.10- 0.01- 20 40 60 80 100 Time (h) A. For the cellulase genes tested place them in one of three categories: (i) essential, (ii) important, or (iii dispensable. For each gene state why you place it in a given category. B. "The cbp2D mutant strain has both a reduced growth rate and maximum level of growth compared to wild-type." Do you agree with this interpretation of the data? Explain your answer. 10

Your lab is investigating cellulose degradation by Cellvibrio japonicus and has generated a large number of mutant strains. You use the wild-type (WT) strain as your positive control and an enzyme secretion mutant (gspD) as your negative control. The remaining eight strains have in- frame deletions for genes predicted to encode cellulases. When you grow the strains on filter paper you obtain the following growth data: Filter paper • WT + gspD cel45A cel6A 0.10- + cel5A + cel5B 0.01- 20 80 Filter paper + WT + gpsD + cbp2D e cbp2E + Ipmo10A * Ipmo10B 0.10- 0.01- 20 40 60 80 100 Time (h) A. For the cellulase genes tested place them in one of three categories: (i) essential, (ii) important, or (iii dispensable. For each gene state why you place it in a given category. B. "The cbp2D mutant strain has both a reduced growth rate and maximum level of growth compared to wild-type." Do you agree with this interpretation of the data? Explain your answer. 10

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter15: From Dna To Protein

Section15.1: The Connection Between Dna, Rna, And Protein

Problem 1SB

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