You've just started your summer intern as a research assistant in the biology department. Your first assignment is to prepare a pH 7.50 phosphate buffer solution to be used in the isolation of DNA from a cell culture. You've discovered the following items available to you in the lab: 0.514M HPO 4 (K a-4.2x10 13) 48.6 g KOH (molar mass - 56.1g) a. Calculate the pH of your buffer. b. Do you think that this would be a suitable buffer? Explain. SHOW YOUR WORK. Upload your file here.
You've just started your summer intern as a research assistant in the biology department. Your first assignment is to prepare a pH 7.50 phosphate buffer solution to be used in the isolation of DNA from a cell culture. You've discovered the following items available to you in the lab: 0.514M HPO 4 (K a-4.2x10 13) 48.6 g KOH (molar mass - 56.1g) a. Calculate the pH of your buffer. b. Do you think that this would be a suitable buffer? Explain. SHOW YOUR WORK. Upload your file here.
Chemistry & Chemical Reactivity
9th Edition
ISBN:9781133949640
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter16: Principles Of Chemical Reactivity: The Chemistry Of Acids And Bases
Section: Chapter Questions
Problem 106GQ: The hydrogen phthalate ion, C8HsO4, is a weak acid with Ka = 3.91 106....
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