The shape and magnitude of the UV spectra depends on the composition of amino acid in each protein. Due to the aromatic amino acid residues in the protein, the observed UV absorbance was mainly in the 240 nm to 340 nm region. In Figure 1 to 3, the maximal absorbance of each protein was approximately at 280 nm. The difference in magnitudes of the peak observed was linked to the differences in the amino acid contents in each of the proteins. The peak of lysozyme was greater than those of BSA and gelatin
chain amino acids, creatine, medium chain triglycerides, pyruvates and vitamins. I. What are Branched chain amino acids? Branched chain amino acids (BCAA) are amino
Hypothesis: If a polar amino acid is adsorbed by a polar matrix, then the polar matrix will not move as far along the matrix as a hydrophobic amino acid. If this is so, the Rf value of a polar amino acid will be relatively small to that of a non-polar amino acid. Introduction: In order to identify whether an amino acid is polar (hydrophilic) or non-polar (hydrophobic), the process of chromatography is used, which separates the amino acids and identifies them based on specific chemical traits given
Branched Chain Amino Acid as a Supplement for Long Duration Exercise Introduction Branched Chain Amino Acids (BCAAs) are composed of three essential amino acids including leucine, isoleucine, and valine. They are unique in that they are predominantly metabolized outside of the liver in the skeletal muscle. BCAAs make up 35-40% of the amino acid pool in the body and exist in high concentrations in skeletal muscle tissue (Shimomura et al, 2006). While the supplemental dosage is dependent upon on
AMINO ACID SEQUENCE ANALYSIS OF THE SUBUNIT B FROM CHOLERA TOXIN PRODUCED BY Vibrio cholerae O139 CELIO CABRAL OLIVEIRA NUCLEOTIDE SEQUENCE ANALYSIS RUTGERS UNIVERSITY FALL 2014 INTRODUCTION Cholera is a tropical disease that causes critical dehydration. The bacterium, which can be found in contaminated water or food, produces the toxin that causes unusual fluid secretion (1). Even though cholera still causes several deaths, about 80% of the cases can be treated with rehydration therapy
Practical Exercise 3 – Buffers and Titration of An Amino Acid Name: Stephanie Tayas Anak Bumphray Id Number: 100084790 Date Performed: 11 September 2017 Practical Group: Monday 1:30pm – 5:30pm Introduction: A buffer solution is which resists large changes in pH when the small amounts of an acid or a base are added to it. The buffer can be calculated based on the equation of ionization pair of acid and conjugate base. Any weak acid can be represented as “HA” and "A-" as the conjugate
Identification of Unknown Amino Acid & Measurements of Buffer Capacity Student #: 998493250 Authors: Young, Lee & Melissa Abstract The titration curve of the unknown exhibited many characteristics, such as equivalence points, pKa of ionizable groups, isoelectric point, and buffer regions, that are particularly distinct to lysine. For unclear reasons, the pH during the titration did not reach the pH for pure 0.2 M NaOH nor 0.2 M HCl and normal equivalence points expected at two extreme
of An Unknown Amino Acid From Titration Abstract Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial pH of the solution was 1.96, and the pKa's found experimentally were 2.0, 4.0, and 9.85. The accepted pKa values were found to be 2.10, 4.07, and 9.47. The molecular weight was calculated to be 176.3 while the accepted value was found to be 183.5. The identity of the unknown amino acid was established to be glutamic acid, hydrochloride
unknown Amino Acids with Acid and Base for Identification Chemistry 315- biochemistry Section 01-L1 Dr. Samantha Iverson Submitted by: Farnaz Imtiaz Submitted to: Mariana Dorrington June 20th, 2017 Introduction – Amino acids are the basic building blocks of the body and sources of energy, like fats and carbohydrates. They are structurally characterized by the fact that they contain nitrogen (N), whereas fats and carbohydrates do not. Therefore, only amino acids are capable
Determination of An Unknown Amino Acid From Titration Abstract Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial pH of the solution was 1.96, and the pKa’s found experimentally were 2.0, 4.0, and 9.85. The accepted pKa values were found to be 2.10, 4.07, and 9.47. The molecular weight was calculated to be 176.3 while the accepted value was found to be 183.5. The identity of the unknown amino acid was established to be glutamic acid, hydrochloride. Introduction