Catalase

referred to as catalase. This particular enzyme speeds up the reaction that specifically breaks down H2O2 into H2O and O2. Catalases, as well as other enzymes, are able to speed up the chemical reaction by lowering the activation energy (Urry, 2016). There are multiple ways to affect how fast a catalase can lower the activation energy during the breakdown of hydrogen peroxide: Tissues of different materials each contain a different genetic makeup, which react differently when exposed to catalase.

concentration of the enzyme catalase in different tissues was also tested. It was tested by pouring 5 ml of hydrogen peroxide over liver in different conditions and over a piece of meat and potato. Enzymes are balls of protein that change a specific substrate into a product by contact at the active site. Enzymes are reusable and once they finish tuning one substrate into a product, it moves on to the next substrate it bumps into in just the right way. The enzyme catalase, which this lab focuses on

The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically, the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required

Introduction This research experiment will fundamentally focus on the activity of catalase, an enzyme that can be found in nearly every living organism. The essential function of catalase is to decompose hydrogen peroxide to produce water and oxygen. This enzyme also plays a major role within the protection of the cell from the oxidative damage that the reactive oxygen species are capable of causing. In addition, catalase is known to have the ability of chemically converting substrates such as hydrogen

at which the catalase enzyme will break down hydrogen peroxide into its products, oxygen and water. Hypothesis If the pH of the solution is related to the reaction rate of catalase, then changing the acidity of the environment will affect the decomposition rate of hydrogen peroxide because catalase has an optimal pH level at which it functions best. Since catalase is found throughout the human body (and highly concentrated in the liver), it is expected that the optimal pH of catalase will be similar

Conclusion: As shown in table 2, the data gathered from the experiment supports the hypothesis that the rate of reaction is highest at pH 7.08 because catalase functions the most efficiently in a neutral environment. It can be seen in the data that the mean value of the rate of reaction is highest at the neutral pH. It shows that catalase breakdown higher amount of hydrogen peroxide into water and oxygen in the pH level of 7. As the pH of the solutions decreased or increased from the optimum level

Discussion: According to the experiment, the highest reaction rate occurred when the enzyme, Catalase, was 50% concentrated (see Fig.3). However, all of the rates were fairly similar no matter the concentration, except for the test with 0% concentration. That test resulted in no production of Oxygen because there was no enzyme for the Hydrogen peroxide to react to so nothing occurred in the testing container (see Fig. 1). In Fig. 2, you can see that all of the concentrations, aside from 0% concentration

and liver increases, the froth produced in the measuring cylinder will increase in volume. As the temperature is increased, the rate at which the catalase breaks down the hydrogen peroxide will increase. Once the optimum temperature of approximately 38°C is reached, the reaction rate will then decrease due to the high temperatures denaturing the catalase enzyme. The averages of both the collated froth volume produced and reaction rate, show that as the temperature increased from 10°C to about 30°C

Hydrogen Peroxide Decomposition Under Varied Temperatures Through Gas Collection The goal of this lab was to determine the effect of temperature on the reaction rate of catalase. We created stock solutions of homogenized chicken liver, controlled the temperature through a water bath, and added the enzyme catalase to the hydrogen peroxide substrate. We then collected the gas through a water displacement setup and recorded how long each trial took to produce 2 ml, 4 ml, and 6 ml of oxygen

Abstract Catalase is an enzyme needed to break down hazardous hydrogen peroxide into nonhazardous oxygen and water. This is needed for organisms as the reaction rate of the decomposition of hydrogen peroxide is too slow to protect cells against the oxidation of hydrogen peroxide. The purpose of this experiment is to test the concentration of hydrogen peroxide on catalase and variation in temperature of catalase to find the optimal values that will produce the highest rate of products. It was hypothesized