Catalysis

With both the stock substrate and varying enzyme solutions prepared, the Spec20 spectrophotometer was used to investigate the enzymatic activity of β-Galactosidase through an absorbance-based assay. Using LoggerPro software on the computer to analyze the absorption data, the Spec20 was calibrated before each run with 0.5 mL of the tested enzyme concentration at an absorbance of 420 nm. Data collection was then started, instantly followed by the addition of 0.5 mL of the stock 2.5 mM substrate solution

The Effects of Increasing Enzyme Concentration on Enzyme Function Purpose The purpose of this lab is to test for enzyme activity by examining factors that may influence enzymes. Background The reaction taking place is catalase breaking down hydrogen peroxide into oxygen and hydrogen. The enzyme is catalase, the substrate is hydrogen peroxide, and the products are hydrogen and oxygen. The question being tested is when we increase the yeast concentration, what will happen to the rate of the reaction

During this experiment, enzyme activity of catecholase and its relationships with enzyme concentration, pH, temperature, and substrate concentration were tested. Enzymes are proteins that speed up biochemical reactions. By using a spectrophotometer, absorbance rates of the various samples were measured and the enzyme activity for each relationship was observed. While we were testing the effects of enzyme concentration, we found that as the concentration of enzymes in solution increased, the higher

The Effect of low pH on Enzyme Activity Frank Keith Welsh, BIO 102, Fall semester Today I will be providing an experiment on the effects of pH on enzymes. Enzymes are affected by changes in pH. Exceptionally high or low pH values commonly cause in complete loss of activity for most enzymes. Furthermore to include temperature and pH there are other elements, such as ionic strength, that can shake the enzymatic reaction. To each of these both physical and chemical parameters should be considered and

Succinate Dehydrogenase and the Inhibitory Effect on its Specific Activity by Malonate By: Danielle Santiago, Nikita Shah, Zachary Voigt, Roseline Oyebanji Introduction This experiment is used to determine the level of succinate dehydrogenase (SDH) activity in a variety of reactions. Mitochondrion from a sample of cow liver is isolated via differential sedimentation and a Bradford Assay is performed to determine the concentration of SDH in the sample (Week 1 Recitation, 2013-2014). The ultimate

Enzymes are protein molecules that are biological catalysts in the human body, meaning that they contribute in the breaking down of certain molecules. Enzymes have a specific active site which the substrate (reactant) that is complementary to will bind with. When the substrate and enzyme bind, an enzyme-substrate complex occurs which means that a reaction takes place. The enzyme acts as a catalyst and breaks the bonds of the substrate. However different enzymes have different optimum pH &temperature

Objective: The purpose of this experiment was to discover the exact change of enzyme activity of catalase and how it corresponded to temperatures, by measuring the rate of appearance of a product. Introduction: Enzymes are substances produced by living organisms, in which act as a catalyst. Their purpose is to trigger a specific biochemical reaction, and provide control of metabolism. Enzymes lower energy barriers, which is why they are able to increase the speed of a cell's chemical reaction.

Introduction: Enzymes are proteins that are essential to our body because it acts as a catalase to speed up chemical reactions within the body using less energy. Enzyme is very specific, it works like a key and lock method by having the exact substrate binding to the enzyme’s active site. Even though enzyme can be reuse to a point that they are denatured. When something is denatured there shape become deform and no longer works. When enzyme doesn’t work our body reactions slows down to a point

My project is a simple experiment to test how enzymes are affected by acidic pH levels. Because enzymes are proteins, they too can be changed by heating, pH level and reagent. Specific enzymes are activated for specific reactions within the body. With the addition of different acids and bases can also affect how a protein is reacted when together. Specifically, if the environment for the enzyme is not properly maintained that it can cause the enzyme to alter such as denature and shutdown. In this

Most enzymes are proteins. They are catalyst that are able to speed up biological reactions by lowering the activation energy needed for the reaction to occur. (BBC, 2011) Enzymes are substrate specific and therefore each enzyme can only fit a certain substrate. They have an active site on the surface of the enzyme into which the substrate binds. Enzymes may break down a single substrate molecule into simpler substances, or join two or more substrate molecules together. As enzyme substrate bonding