E. coli

Antiseptics play an important role in preventing microbial growth and killing microorganisms. They are used in many fields such as healthcare fields and hospitals as agents to prevent infections. To observe the effectiveness of disinfectants and antiseptics on certain organisms you would use the Kirby-Bauer. The Kirby-Bauer is used as disk diffusion method as seen in Figures 1 and 2. This experiment used filter discs that were immersed in the antiseptics and were placed on the Tryptic soy agar plate

substance and can be used as a tag when fused to a protein of interest. The purpose of this experiment was for us to determine which culture (A and B) was treated with Isopropyl B-D-thiogalactoside (IPTG) and therefore would express GST. The bacteria E. coli BL21 carrying the pGEX-2T plasmid was used to express GST protein after which protein extraction and GST purification from other proteins where done. GST expression is controlled by the Lac operon promoter (Plac). The Lac promoter sequence drives

It’s surprising when the body immune attacks its own body immune system instead of defending from disease. Systemic lupus erythematosus (SLE) is one of the diseases that attacked by the body immune system: how the immune system begins attacking its own body was unknown. According to a recent research finding in Temple University School of Medicine (TUSM), lupus disease has been caused by bacterial communities in the gut or biofilm. “TUSM researchers Çagla Tükel, PhD, and Stefania Gallucci, MD

Pyelonephritis is the inflammation of the kidney that is mostly caused by a bacterial infection, which leads to scarring of the kidney. It is a form of a urinary tract infection (UTI) one instance may lead to substantial renal damage, kidney failure, abscess formation, sepsis, septic shock, and the failure of multiple organs. The infection typically begins in the urethra or bladder and travels up into the kidneys, where it could potentially harm the organs and even the life of the infected individual

“The Plastic Surgery of Food” This year more than 559,000 Americans will die of cancer, and another 1,445,000 new cases will be detected. (Kushi, Jack 3) According to the American National Cancer Society, facts and figures from 2017 has linked that sixty years ago the rate of people who would get cancer in their lifetime was one of eight, however, today it has risen to one in two men and one in three women. Several studies indicate processed foods are a leading contributor to the rising cancer

SP-D had shown the growth inhibition of E coli, Legionella pneumonia, Enterobacter aerogenes and Klebsiella pneumonia by increasing the cell membrane permeability (Wu et al., 2003) but not in the case of mycobacteria (Ariki, 2011). We performed experiment to find out either recombinant conglutinin increased the cell membrane permeability of the M. bovis BCG and as a result growth inhibited which is shown in figure 4.3. Heat-treated M. bovis BCG used as a positive control. While heat-treated M. bovis

Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The

Mark one tube “+” and the other “-”. The “-” tube will include the plasmid. Using a sterile graduated pipet, add 0.25 mL ice cold calcium chloride to each tube. Hold those tubes on ice. Obtain a starter plate of E. coli and using a sterile inoculating loop to transfer one large colony of bacteria form the starter plate to each tube of cold calcium chloride. In order to remove the bacteria from the transfer loop and break them apart, place the loop into the calcium

The purpose of this experiment was to find the range assay for determining the activity of the E. Coli enzyme, Beta-galactosidase. An enzyme assay is how enzyme activity is measured in a lab. It is useful to know the activity of a given preparation of an enzyme in order to know how much of the enzyme solution is needed for each reaction tube to produce a suitable reaction rate. Enzyme activity is also necessary in order to study enzyme kinetics and inhibition. To start, enzymes are biological catalysts

coli cells. Removal of all or part of the non-essential region, between positions 20-35 using restriction enzyme HindIII decreases the size of the resulting molecule by up to 15 kb (Brown, 2006). Since during this experiment that bacteriophage DNA molecule