E. coli

rare proteins with high fluoresce and absorbance. The purpose this experiments is to purify and express a His2-tagged recombinant from of GFP (rGFP) from the E. coli strain BL21(DE3)< pRSETA-GFPUV > through a series of experiments by using Ni+2 agarose affinity chromatography technology. The GFPuv gene (UV-optimized GFP) was over expressed in the E. Coil strain BL21 (DE3) (pLysS) as an n-terminal His6/Xpress epitope tagged bind protein. Then using Ni2+ Agarose affinity chromatography to obtain purification

tetracycline resistance making it difficult to keep the bacteria from growing. We learned that “reports of severe gastroenteritis linked to eating raw or undercooked meat have led FDA to investigate possible sources of the contamination.” Escherichia Coli bacteria was found in the batched

The domestication of animals 30,000 years ago was the precursor to Genetic Engineering. Starting with the dog we have since been able to introduce desirable traits in all organisms. With the discovery of plasmids in the late 60’s we have been able to take genetic engineering even further. Plasmids are small circular DNA molecules used to amplify and replicate a gene of interest. These minute molecules have the ability to replicate with the chromosome or independently, allowing them to have up to

Introduction: Infections are the major causes of mortality and morbidity in the intensive care unit (ICU). More than 50% of patients admitted to ICU were infected, 71% of these patients received antibiotic Infections increase with long stays in the ICU. The infection rates more than 70% in patients stay more than 7 days. (1) ICU patients have high risks Nosocomial gram negative bacterial infections, which associated with high mortality and morbidity rates. The most common bacteria isolated

available that maps the types of bacteria commonly found on fruits and vegetables (Leff and Fierer 2013). Many different standards have been set forth to measure produce quality with some of these standards including a count of the coliforms, Escherichia coli, and the aerobic bacteria count (Wood et. al). This study aims to compare the number of coliforms found on organic vs. conventionally grown Malus pumila before and after washing with distilled water.

Companies and organizations of all types all face a common challenge: to evaluate how they want to be seen against the reality of how they are actually seen by various stakeholder groups and publics. How a company wants to be seen is known as its corporate vision. The corporate vision of a company explains the long-term goals and ambitions of a company. A company’s reputation, however, is how a company is seen overall, compared to its nearest rivals. Companies are able to establish strong reputations

Urinary tract infection (UTI) is a microbial infection of the kidneys, ureters, bladder or urethra. Urinary tract is the most common form of infection. Resistance to the extended-spectrum cephalosporins can occur in Klebsiella species due to production of the extended-spectrum ß-lactamases (ESBLs) that make hydrolysis of the oxyiminocephalosporins and monobactams. The aim of the present work was intended to be a prospective study of the plasmid features of Klebsiella isolates, obtained from hospitalized

Mechanisms of Resistance Mechanisms of Antibiotic Resistance The principle of antibiotic resistance revolve around how antibiotics work. Antibiotics target certain structures on bacteria such as their cell wall, proteins, and nucleic acids that results in the disruption and/or inhibition of their growth. These disturbances can sometimes lead to bacterial death. In order to survive, bacteria have developed countermeasures to fight against the harmful drugs. This was carried out by targeting the antibiotics

No other aquatic organism like the jellyfish instills fears in ocean swimmers. Known for their graceful movements, tentacles, and stinging ability, the jellyfish is avoided at all cost. There is, however, a particular species of jellyfish Aequorea Victoria that has been very beneficial to scientists. This species of jellyfish produces green bioluminescence from small photo organs located on its umbrella. In the 1960, while working at Princeton University, the scientist Osamu Shimomura was attempting

Gene BglA was sub-cloned in pET21a (+), recombinant vector transformed and propagated in E. coli BL21 CodonPlus under the control of T7/lac promoter system. The positive bacterial transformants were grown in LB-ampicillin broth (100 µg mL−1) and induced with IPTG for the production of recombinant BglA enzyme. Optimal heterologous expression and efficient folding of BglA protein was observed with 0.5 mM IPTG, after 72 h incubation at 22°C. The use of reduced concentration of IPTG for getting maximum