Silica gel

Figure 1 shows a standard curve developed from the cytoplasmic protein standards of lung cancer cells. A gradual increase in corrected absorbance can be notes, which more noticeably increases after 125 μg/mL. After this point the values nearly double. The absorbance readings for the samples were recorded along with the standard concentrations [500 μg/mL, 250 μg/mL, 125 μg/mL, 62.5 μg/mL, and 0 μg/mL (PBS only) 
The corrected absorbance for the protein standards were found by subtracting the average

an unknown gene. The unknown gene is then ligated into a vector plasmid, which is placed in a bacterium Escherichia Coli to transform and multiply. Ultimately, identification and characterization of the unknown gene is done using electrophoreses and gel imaging. Cloning techniques such as the one performed have been used for many years to isolate genes from a variety of species. Isolation of unknown genes serves many purposes. Nucleic acid sequence of the unknown gene can be derived which can be compared

These proteins have a hierarchy of structure that consists of folding that determines the direct function of each protein.. The molecular weight of these proteins were measured using SDS PAGE. SDS PAGE stands for sodium dodecylsulfate polyacrylamide gel electrophoresis. SDS is an anion detergent that binds with the protein structures and causes them to separate due to the change in bonding charge. SDS and heat are how the proteins are denatured. The process of denaturing a protein is breaking

testing the DNA of our two suspects, and the DNA sample from the crime scene. My lab assistants and I will be using an agarose gel to analyze the DNA. We will first dissolve the agarose powder by heating it in a buffer, and then pouring it into a casting tray to harden. Small pores will form as the gel hardens, and they will act to separate the DNA samples. Next, the cooled gel is placed into an electrophoresis chamber. It will be covered by a 1xT TAE buffer. We will load the DNA samples into the compartments

separated on the basis of the size since all are the same molecule (Gel electrophoresis). How does it work? The gel is used to hold DNA together, it is made up of polysaccharide which is called agarose. The molecules of a gel are held together by hydrogen bonding and form tiny pores. DNA samples are placed in the wells; which is a tiny pocket in the gel(Gel Electrophoresis). Electric current is passed through the gel so one end of the gel is positive charge and another end is a negative charge. In this

standards were separated alongside our protein samples which allowed determination of the sizes of our protein in our sample. SDS denature proteins and coats them with a negative charge allowing for their electrophoretic separation by polyacrylamide gel electrophoresis (PAGE). As you can see, after being stained with the coomassie blue, we could detect proteins of various sizes and our unknown protein expressing GST was detected in our Pure B sample (Figure 2). The distance travelled from the wells

pigmentation on the gel. On the first well, it is found the normal hemoglobin that showed only one band. The normal hemoglobin has one polypeptide chain and one heme group and carry the same heme group iron that are associated with polypeptide chain of 141 (alpha) and 146 (beta) amino acids residues (Marengo, 2006). The normal hemoglobin have two kinds of chain known as alpha and beta chains.

fingerprinting known as gel electrophoresis. We used substitute chemicals instead of DNA to demonstrate the process of this. electrophoresis is the process in which DNA can be analyzed using tandem repeats. These are the 4 base pairs that repeat in the same order for a certain number of times. The number of times these base pairs repeat can be seen when the DNA is run through the electrolysis chamber. The DNA is loaded into a gel well then a current is run through the buffer that the gel is submerged in

Plagiarism will result in an automatic zero. 1. In the cell bio lab, we use company manufactured gels, however you can make you own polyacrylamide gels. List all of the ingredients found in an SDS-PAGE gel. Which ingredients are responsible for polymerizing the solution? How does the percentage of acrylamide effect the migration of proteins (ex: 4% gel vs. 18% gel)? The percent

Introduction To explain nanocrystalline materials, it should have been explained that “nano” concept at first. Nanostructures are relatively new and interesting subject to investigate, they are in a scale which is between 0-100 nanometers (1 nanometer, nm is equal to 10-9 meter). There are some types of nanostructured materials such as lamellar (1-dimensional), filamentary (2-dimensional) and crystallites (3-dimensional). If grains of the material are made up of crystals, then “nanocrystalline materials”