Sol-gel

standards were separated alongside our protein samples which allowed determination of the sizes of our protein in our sample. SDS denature proteins and coats them with a negative charge allowing for their electrophoretic separation by polyacrylamide gel electrophoresis (PAGE). As you can see, after being stained with the coomassie blue, we could detect proteins of various sizes and our unknown protein expressing GST was detected in our Pure B sample (Figure 2). The distance travelled from the wells

pigmentation on the gel. On the first well, it is found the normal hemoglobin that showed only one band. The normal hemoglobin has one polypeptide chain and one heme group and carry the same heme group iron that are associated with polypeptide chain of 141 (alpha) and 146 (beta) amino acids residues (Marengo, 2006). The normal hemoglobin have two kinds of chain known as alpha and beta chains.

methods available for the separation or isolation of organic compound from a mixture. The most common used chromatographic technique includes column chromatography, thin -layer chromatography (TLC), etc. The purpose of this lab exercise is using the gel filtration chromatography to separate different proteins from a mixture. Collecting elutes (run off), and calibrating a curve to show how the column separates molecules by molecular weight, and identify the molecular weight of the proteins. The sample

fingerprinting known as gel electrophoresis. We used substitute chemicals instead of DNA to demonstrate the process of this. electrophoresis is the process in which DNA can be analyzed using tandem repeats. These are the 4 base pairs that repeat in the same order for a certain number of times. The number of times these base pairs repeat can be seen when the DNA is run through the electrolysis chamber. The DNA is loaded into a gel well then a current is run through the buffer that the gel is submerged in

In this experiment, the objective was to use thin layer chromatography to identify the major active ingredients in commercial analgesic preparations. TLC is a method to separate compounds and to see how many compounds are present in the mixture. The separation into components is also dependent on the solvent used. When TLC is performed, the Rf values are determined for the sample and they are compared to the Rf values of the standards. Similar Rf values help identify the standards that might be

The TLC (Thin-Layer Chromatography) will be used to determine the composition of various over-the-counter analgesic drugs. By doing so, the identities of actual trade names of the unknown analgesics can be found, almost mimicking the other procedures done in this laboratory with a similar outcome: finding the unknown’s identity through comparing and analyzing. Experimental First off, an amount of at least 12 capillary micropipettes was needed in this experiment to spot the plates.,To create capillary

Plagiarism will result in an automatic zero. 1. In the cell bio lab, we use company manufactured gels, however you can make you own polyacrylamide gels. List all of the ingredients found in an SDS-PAGE gel. Which ingredients are responsible for polymerizing the solution? How does the percentage of acrylamide effect the migration of proteins (ex: 4% gel vs. 18% gel)? The percent

Protein Composition in Red Blood Cells in Humans using Polyacrylamide Gel Electrophoresis Name: Emma Claypole Date: Wednesday March 16, 2016 Lab Group: W08, Wednesday morning 2 Abstract The proteins of Bovine red blood cell (RBC) membranes were analyzed using polyacrylamide gel electrophoresis. After analyzing Bovine RBC they were then compared to human RBC counterpart. Following finding the log of each molecular weight of each band, band one showed the highest molecular weight. All five

Attenuation effect of caffein and/or nifedipine against selenite-induced cataractogenesis Abstract Objectives: The current study was performed to investigate the anti-cataractogenic effect of single and combined treatment with caffeine and nifedipine in sodium selenite induced cataract. Methods: Seventy five healthy albino Wistar rats were divided into 5 groups; the first group received intraperitoneal injection of saline and served as control, 2nd group received a single subcutaneous injection

and references, expanded on experimental errors in more detail and ensured my aim and conclusion is clear. Abstract The purpose of this investigation was to identify the class of immunoglobulins using, sodium dodecyl sulphate (SDS) Polyacrylamide Gel Electrophoresis (PAGE); by making deductions about the structure and molecular organisation of the protein. The experiment was conducted to calculate the unknown molecular mass for reduced and non-reduced immunoglobulin, using SDS-PAGE by measuring